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Stemcell  60012FI.1  Anti-Human CD32 Antibody, Clone IV.3

Product Overview

The IV.3 antibody reacts with human CD32 (FcγRII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation and platelet aggregation. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes/macrophages, placental trophoblasts and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the IV.3 antibody may be employed as a blocking antibody to reduce non-specific binding. The IV.3 antibody binds most strongly to the IIa isoforms of CD32, with the epitope mapped to amino acids132-137 [FSHLDP] in domain 2, within the ligand binding site.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051) and EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052).

Subtype:一抗

Target Antigen:CD32

Reactive Species:人

Conjugation:FITC; 未结合

Host Species:小鼠

Cell Type:B细胞; 粒细胞及其亚型; 单核细胞

Application:细胞分离; 流式细胞术; 功能检测; 免疫细胞化学; 免疫组化; 中和及阻滞; Western印迹法

Area of Interest:免疫学

Clone:IV.3

Gene ID:2212

Isotype:IgG2b, kappa

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