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Overview
ClonaCell™ FLEX is a base methylcellulose medium that requires the addition of a 2X concentrate of the user’s own liquid medium to create a custom semi-solid cloning medium. This allows the user to adapt an existing liquid cloning method to a customized semi-solid cloning method. ClonaCell™ FLEX is recommended for selection and cloning of suspension-adapted mammalian cells, including CHO cells and hybridomas. The medium is chemically defined, protein-free and animal component-free. It does not contain L-glutamine or selection agents. The medium is compatible with a wide range of selection systems, including dihydrofolate reductase (DHFR), glutamine synthase (GS), and hypoxanthine-aminopterin-thymidine (HAT).
Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.
• Flexibility to create a custom semi-solid medium optimized for cloning a wide variety of mammalian or non-mammalian cell types
• Water