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Overview
ClonaCell™-TCS Medium is a serum-containing methylcellulose-based semi-solid medium that can be used for selecting and cloning a variety of suspension-adapted cell lines, including CHO-S and hybridomas. It can also be used for semi-solid cloning of some cell lines that grow adherently in the presence of serum, including CHO-K1, BHK-1, and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA) and supports robust growth of a wide variety of cell types. ClonaCell™-TCS Medium does not contain selection agents.
Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.
• Pre-selected serum
• Bovine serum albumin
• L-Glutamine
• 2-Mercaptoethanol
• Methylcellulose
• Other ingredients