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Overview
ClonaCell™-CHO ACF is a methylcellulose-based semi-solid medium with supplements for the selection and cloning of suspension-adapted CHO cells in serum-free conditions. This medium is animal component-free and contains only recombinant proteins and synthetic components. Single-cell cloning efficiency of CHO cells is higher in this medium than in protein-free media, especially at low plating densities. It does not contain L-glutamine or selection agents and is compatible with dihydrofolate reductase (DHFR) and glutamine synthase (GS) selection systems.
Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.
• Greater single-cell cloning efficiency of CHO cells compared with chemically defined, protein-free media, particularly at low plating densities
• Recombinant proteins (animal-component free)
• Other ingredients
• Does not contain L-glutamine or selection agents