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Overview

ClonaCell™-CHO CD Medium is a chemically defined, protein-free semi-solid medium designed for selection and cloning of suspension-adapted CHO cells. This methylcellulose-based medium does not contain L-glutamine, selection agents, or animal-derived components. ClonaCell™-CHO CD Medium is compatible with dihydrofolate reductase (DHFR) and glutamine synthase (GS) selection systems.

Benefits of semi-solid cloning:

• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.

• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.

• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.