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Stemcell  18063  EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit

Product Overview

This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™ . Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

Advantages:• Highly purified human CD4+CD127lowCD25+ Tregs isolated in less than 1 hour

• No-wash removal of EasySep™ Releasable RapidSpheres™

• Optional isolation of CD4+CD25- responder T cells from the same sample

Components:

EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)

EasySep™ Human CD25 Positive Selection Component A, 1 mL

EasySep™ Human CD25 Positive Selection Component B, 1 mL

EasySep™ Human CD127high Depletion Cocktail, 1 mL

EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL

EasySep™ Releasable RapidSpheres™, 1 mL

EasySep™ Dextran RapidSpheres™, 2 x 1 mL

EasySep™ Release Buffer, 2 x 1 mL

Magnet Compatibility:

• EasySep™ Magnet (Catalog #18000)

• “The Big Easy” EasySep™ Magnet (Catalog #18001)

Subtype:细胞分选试剂盒

Cell Type:T细胞; CD4+T细胞; 调节性T细胞

Species:人

Sample Source:白细胞去除术; PBMC

Selection Method:负选

Application:细胞分离

Brand:EasySep

Area of Interest:免疫学

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