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Roche 04942078001 Dispase II (neutral protease, grade II)

公司简介

罗氏总部位于瑞士巴塞尔，在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一。作为全球最大的生物技术公司之一，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统领域拥有切实有效的差异化药品；同时罗氏也是体外诊断和基于组织的肿瘤诊断的市场领导者，以及糖尿病管理领域的先驱者。罗氏倡导的“个性化医疗”方案旨在提供创新的药品和诊断工具，从而切实改善人类的健康水准、生活质量以及患者的生存期。

在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。

产品介绍

Features and Benefits

• Rapid, effective, yet gentle agent that liberates cells with minimal cell damage• Maintains cell membrane integrity• Non-mammalian source - free of mycoplasma and animal virus contamination• Extremely stable to influences of temperature, pH, and interference by serum components• Easily inactivated by chelating agents or by dilution• Delivers higher activity and convenience

Other Notes

仅用于生命科学研究。不可用于诊断。

Preparation Note

Activator: Optimal Ca²⁺ concentration is 2 mM. The enzyme preparations contain enough Ca²⁺ for optimal activity.Inhibitors: EDTA, EGTA, Hg²⁺, other heavy metals. Dispase is not inhibited by serum.Working concentration: 0.6 to 2.4 U/mlWorking solution: Preparation of stock and working solutions:To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase^® II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.Storage conditions (working solution): -15 to -25 °CThe reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.

Specificity

Dispase is a nonspecific metalloprotease.

Unit Definition

One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 μmol (181 μg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).A practical comparison of Roche units of Dispase^® II with those cited in the Japanese literature (where Dispase^® concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.One unit of Roche Applied Science Dispase^® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.

Application

Dispase^® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture).^[2][4][1] Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.

General description

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.^[3]

法律信息

Dispase is a registered trademark of Godo Shusei Co., Ltd.

性质

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