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BD  Pharmingen™  553013  FITC Rat Anti-Mouse CD90.2  Clone  30-H12  (RUO)

产品编号  553013|BD Pharmingen™

产品描述

Brand：BD Pharmingen™

Alternative Name：Thy-1.2

Concentration：0.5 mg/ml

Isotype：Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ

Reactivity：Mouse (QC Testing)

Application：Flow cytometry (Routinely Tested)

ImmunogenMouse：Thymus / Spleen

Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status:RUO

规格货号

553013

目录价

¥ 3,263.00

单位 EA (1 Each)

产品详情

DESCRIPTION

The 30-H12 clone has been reported to react with the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. Thy-1.2 has also been initially reported to be detectable on thymic dendritic cells, but later revealed that the antigen was probably picked up from T-lineage cells. 30-H12 mAb has been reported not to cross-react with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that crosslinked 30-H12 antibody induces Ca2+ influx into thymocytes and that co-crosslinking of 30-H12 mAb with antibody to the CD3/TCR complex intensifies thymocyte signal transduction, promotes apoptosis of thymocytes, and inhibits the CD3-mediated proliferative response of mature T lymphocytes.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

FORMAT

Format:FITC

Excitation Source:Blue 488 nm

Excitation Max:494 nm

Emission Max：520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Other Formats →

  BB700

  BUV395

  BV421

  BV510

  BV605

  BV650

  BV711

  BV786

  Biotin

  NA/LE

  PE

  Purified

SUGGESTED COMPANION PRODUCTS

RESOURCES & TOOLS

  Spectrum Viewer

  Panel Designer

  Download TDS

PREPARATION AND STORAGE

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

PRODUCT NOTICES

Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

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