Clone: | Nestin 20 (See other available formats) |
Isotype: | Rabbit Polyclonal |
Reactivity: | Human, mouse |
Immunogen: | The polyclonal nestin antibody was raised using a KLH conjugated peptide to the mouse nestin tail. |
Preparation: | Serum |
Concentration: | Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.) |
Storage & Handling: | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. For long-term storage, keep antibody at -80°C. |
Application: | WB, IF, IHC, ICC |
Recommended Usage: | Each lot of this antibody is quality control tested by immunocytochemistry.The optimal working dilution should be determined for each specific assay condition.• WB: 1:1,000*• IF: 1:200-500• IHC: 1:200-500 |
ApplicationNotes: | This antibody is effective in immunoblotting, immunofluorescence and immunohistochemistry.*Predicted MW= 220-240 kDTissues: Formalin fixed paraffin-embedded tissues.This antibody was tested using Ntera-2 cells and mouse embryonic cells and can be used for Western blotting and immunofluorescence. |
ApplicationReferences: | 1. Gaspard N, et al. 2009. Nat Protoc.4:1454.2. Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed 3. Shimada IS<et al. 2011. Stroke. 42:3231. (WB) 4. Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC) PubMed 5. Del Valle L,et al. Cancer Biol Ther.9:286. (IHC) PubMed 6. Boraas L, et al. 2016. Plos One.. 12: 1005399. PubMed |
Publication Library | |
RRID: | AB_2565443 (BioLegend Cat. No. 839801) |
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