Anti-HA.11 Epitope Tag Affinity Matrix

￥ 7560.00

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Biolegend(Covance) 900801 Anti-HA.11 Epitope Tag Affinity Matrix

产品编号 900801| Biolegend(Covance)

产品描述

Clone:	16B12 (See other available formats)
Isotype:	Mouse IgG1, κ
Reactivity:	YPYDVPDYA Tag
Immunogen:	Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation:	Purified IgG immobilized on Sepharose™ Fast Flow beads (in PBS + 0.03% Thimerosal).
Preparation:	The antibody was purified by affinity chromatography.
Concentration:	2 mg/ml
Storage & Handling:	Store between 2-8°C. The matrix may be re-used several times. To strip column after use, wash with several bead volumes of 0.1 M glycine pH 2.8 followed immediately by PBS containing 0.3% Thimerosol or 1mM Sodium Azide as preservative.
Application:	Purification
Recommended Usage:	Each lot of this antibody is quality control tested by immunoprecipitation.The optimal buffers and matrix concentration should be determined for each specific assay condition.Binding: Tagged protein will bind to matrix in common physiologic buffers with pH in the range of 6.0-7.5, salt from 50-500 mM and in the presence of reasonable levels of detergent. Excess reducing agent should be avoided as the disulfide bridges holding antibody heavy and light chains may be compromised. BioLegend tests Affinity Matrix using an equilibration/binding buffer containing:• 100 mM Tris-HCl (pH 7.5)• 150 mM NaCl• 0.1% Tween 20• 0.5% BSA• 1 mM beta-mercaptoethanolWashing: After binding, washes with several bead volumes of buffer are recommended. Such buffer may contain increased salt, altered pH, etc. as determined empirically to remove un-tagged proteins.Elution: Several options are available for elution.1. SDS gel loading buffer may be applied directly to the beads in order to display all bound protein on a polyacrylamide gel/western. Note that gel loading buffer containing reducing agent will also release some antibody heavy and light chains (approx 25 and 50kD, respectively).2. Competitive elution with epitope peptide. For epitope tag affinity matrices, prepare an elution buffer with epitope tag peptide at 400 ug/mL in 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM EDTA (pH 8.0).3. Chemical Elution. Elution by pH or chaotropic salts is also possible. For elution by pH, either 0.1 M glycine pH 2.8 or 40 mM diethyl-amine pH 11.0 may be used.
ApplicationNotes:	This affinity matrix can be used for immunopurification of HA-tagged fusion proteins from crude starting material. On an analytical scale, it can also be used for immunoprecipitating HA-tagged proteins.Monoclonal mouse antibody HA.11 recognizes the peptide epitope, YPYDVPDYA. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.HA.11 antibody was purified using protein-G chromatography and was subsequently immobilized onto a Sepharose™ Fast Flow matrix.Sepharose is a trademark of Amersham Biosciences Limited
ApplicationReferences:	1. Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685. 2. J Field, et al. 1988. Mol Cell Biol. 8:2159. 3. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)PubMed4. Mitxelena J, et al. 2016. Nucleic Acids Res..10.1093/nar/gkw146. PubMed5. Dickey D, et al. 2016. J. Biol. Chem.. 291: 11385 - 11393.PubMed
	Publication Library
RRID:	AB_2564999 (BioLegend Cat. No. 900801)

公司简介

Covance主要业务是研发和生产各种抗体，涉及领域包括：神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。特色产品是免疫组化的体外诊断试剂。美国 BioLegend 公司于圣地亚哥时间2014年8月29日收购了位于马萨诸塞州戴德姆的科文斯（Covance）旗下的科文斯抗体服务公司（Covance Antibody Services Inc.）。科文斯抗体服务公司主要业务是研发和生产各种抗体，涉及领域包括:神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。这些用于免疫组化的体外诊断试剂（IVD）对于 BioLegend 公司目前的科研试剂产品线是一个很好的补充和扩展。科文斯抗体的客户将享有与 BioLegend 当前客户同样的优质服务和高性价比的产品。

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市场价

￥7560.00

价格

￥7560.00

品牌

Biolegend(Covance)

型号

900801