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Biolegend(Covance) 838001 Anti-O-GlcNAc Antibody
产品编号 838001| Biolegend(Covance)
产品描述
Clone: | CTD110.6 (See other available formats) |
Isotype: | Mouse IgM |
Reactivity: | All Species |
Immunogen: | The CTD110.6 antibody was raised against a synthetic peptide containing Serine-O-GlcNAc. |
Preparation: | Ascites |
Concentration: | The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml. |
Storage & Handling: | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
Application: | WB, ELISA, IPICC, IF - Reported in the literature |
Recommended Usage: | Each lot of this antibody is quality control tested by Western blotting.The optimal working dilution should be determined for each specific assay condition.• WB: 1:1,000*• IP: 1:2,500 |
ApplicationNotes: | This antibody is effective in ELISA, immunoblotting (WB) and immunoprecipitation (IP).*Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.This monoclonal antibody represents a powerful new tool for the study of O-GlcNAc's role in cell physiology. This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. |
ApplicationReferences: | 1. Anthonisen EH, Berven L, Holm S, Nygård M, Nebb HI, and Grønning-Wang LM. Nuclear Receptor Liver X Receptor Is O-GlcNAc-modified in Response to Glucose. J. Biol. Chem.; 285: 1607 - 1615, 2010. [WB] PubMed 2. Kneass ZT, Marchase RB. Neutrophils exhibit rapid agonist-induced increases in protein-associated O-GlcNAc. J. Biol. Chem. 279(44):45759-45765, 2004.3. Kim SJ, Sung JY, Um JW, Hattori N, Mizuno Y, Tanaka K, Paik SR, Kim J, Chung KC. Parkin cleaves intracellular alpha-synuclein inclusions via the activation of calpain. J. Biol. Chem. 278(43):41890-9, 2003.4. Comer FI, Vosseller K, Wells L, Accavitti MA, Hart GW. Characterization of a mouse monoclonal antibody specific for o-linked n-acetylglucosamine. Anal. Biochem. 293(2):169-177, 2001.5. Comer FI, Hart GW. O-Glycosylation of nuclear and cytosolic proteins. Dynamic interplay between O-GlcNAc and O-phosphate.J. Biol. Chem. 275(38):29179-29182, 2000.6. Hart GW, Greis KD, Dong LY, Blomberg MA, Chou TY, Jiang MS, Roquemore EP, Snow DM, Kreppel LK, Cole RN, et al. O-linked N-acetylglucosamine: the "yin-yang" of Ser/Thr phosphorylation? Nuclear and cytoplasmic glycosylation. Adv. Exp. Med. Biol. 376:115-123, 1995.7. Dias WB, et al. 2009. J. Biol. Chem. 32:21327. (WB) PubMed 8. Wang Z, et al. 2007. Mol. Cell Proteomics 8:1365. (IP) 9. Kim SJ, et al. 2003. J. Biol. Chem. 43:41890. (WB) 10. Kneass ZT and Marchase RB. 2004. J. Biol. Chem. 44:45759.(WB, ICC, IF) |
Publication Library | |
RRID: | AB_2565386 (BioLegend Cat. No. 838001)AB_2565387 (BioLegend Cat. No. 838002) |
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Covance主要业务是研发和生产各种抗体，涉及领域包括：神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。特色产品是免疫组化的体外诊断试剂。美国 BioLegend 公司于圣地亚哥时间2014年8月29日收购了位于马萨诸塞州戴德姆的科文斯（Covance）旗下的科文斯抗体服务公司（Covance Antibody Services Inc.）。科文斯抗体服务公司主要业务是研发和生产各种抗体，涉及领域包括:神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。这些用于免疫组化的体外诊断试剂（IVD）对于 BioLegend 公司目前的科研试剂产品线是一个很好的补充和扩展。科文斯抗体的客户将享有与 BioLegend 当前客户同样的优质服务和高性价比的产品。
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