综合电商模板

Biolegend  420601 Brefeldin A Solution (1,000X)

产品编号  420601| biolegend

产品描述

Product Details
Formulation:	Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration:	5.0 mg/ml
Storage & Handling:	Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Application:	ICFC - Quality tested
Recommended Usage:	Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.
Application	Completely thaw the solution before use, DMSO freezes at 4°C.  We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.
Notes:
Application	1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
References:	2. Fuse S, et al. 2007. J. Immunol. 178:5227.
	3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
	4. Redfern CH,et al.2006.J. Clin Oncol.19:3107. PubMed
	5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
	6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
	7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
	8. Miner MD, et al. 2008. Microb Pathog. 45:273. PubMed
	9. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
	10. Markey KA, et al. 2009. Blood 113:5644. PubMed
	11. Wilcox RA, et al. 2009. Blood   PubMed
	12. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
	Publication Library
Antigen Details
Description:	Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.
Other Names:	BFA
Antigen	1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
References:	2. Sander B, et al. 1991. Immunol. Rev. 119:65.
	3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
	4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
	5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.
GeneID:	NA
Keywords:	Brefeldin A Solution (1,000X), Solution, BFA, Intracellular Staining for Flow Cytometry, Immunology, Antibodies

公司简介

Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！

Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！

流式细胞术简介：流式细胞术就是利用流式细胞仪对处在快速直线流动状态中的单列细胞或生物颗粒进行逐个、多参数、快速的定性、定量分析或分选的技术，具有检测速度快、测量指标多、采集数据量大、分析全面、方法灵活等特点。流式细胞术已被众多的研究人员作为优先选择的研究技术。目前，流式细胞术已广泛应用于免疫学、细胞生物学、遗传学、生物化学、肿瘤学、血液学等基础学研究和临床医学领域。

目前，Biolegend公司是世界上主要的流式抗体生产商，Biolegend公司作为后起之秀，以其过硬的质量、更低的价格、丰富的规格、优质的服务及快速的到货期为越来越多的科研工作者所青睐！

Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068