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Biolegend 421403 FOXP3 Fix/Perm Buffer Set
产品编号 421403 | biolegend
产品描述
Storage & Handling: | The FOXP3 fix/perm buffer set solution should be stored between 2°C and 8°C. Do not freeze. This set is guaranteed for three months. |
Application: | |
Recommended Usage: | The FOXP3 Fix/Perm buffer(4X) must be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer(4X) to three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Perm buffer(10X) to nine (9) parts PBS. |
NOTE: The FOXP3 Perm buffer (10X) may have crystalization or precipitation oberserved when it is stored at 2-8°C, however, it's normal and does not affect the buffer performance. If there is a heavy precipitation observed after diluted to 1X working solution, it can be filtered to clarify the solution. | |
Application | |
Notes: | |
FOXP3 Intracellular Staining Procedures: | |
2. Add 1 ml of 1X BioLegend's FOXP3 Fix/Perm solution to each tube, vortex and incubate at room temperature in the dark for 20 minutes, then spin down the cells and remove the supernatant. | |
3. Wash once with cell staining buffer (Cat. No. 420201) by spin at 250Xg for 5 minutes and remove the supernatant. | |
4. Wash once with 1ml 1X BioLegend's FOXP3 Perm buffer. | |
5. Re-suspend cells in 1ml 1X BioLegend's FOXP3 Perm buffer, incubate at room temperature in the dark for 15 minutes, spin down cells and discard the supernatant, then resuspend the pellet in 100 ul of 1X BioLegend's FOXP3 Perm buffer. | |
6. Add appropriate amount of flurochrome conjugated anti-FOXP3 antibody and incubate at room temperature in the dark for 30 minutes. | |
7. Wash twice with cell staining buffer, and resuspend in 0.5ml cell staining buffer then analyze with flow cytometer with appropriate instrument setting. | |
Application | |
References: | |
5. Müller M, et al. 2007. J. Immunol. 179:2774. | |
Antigen Details | |
Description: | BioLegend's FOXP3 Fix/Perm Buffer Set has been specially formulated for intracellular staining FOXP3 with minimum effect on the surface fluorochrome staining. It contains one 30 ml 4X concentrated FOXP3 Fix/Perm buffer (Cat. No. 421401) and one 25 ml 10X concentrated FOXP3 Perm buffer (Cat. No. 421402). The FOXP3 Fix/Perm buffer(4X) must be freshly diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer(4X) to three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures.e.g. Dilute one (1) part FOXP3 Perm buffer(10X) to nine (9) parts PBS. |
Caution: The FOXP3 Fix/Perm buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contact. | |
Other Names: | FOXP3 Fixation/Permeabilization Buffer |
GeneID: | NA |
Keywords: | FOXP3 Fix/Perm Buffer Set, Solution, FOXP3 Fixation/Permeabilization Buffer, Intracellular Staining for Flow Cytometry, Immunology, Antibodies |
公司简介
Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！
Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
流式细胞术简介：流式细胞术就是利用流式细胞仪对处在快速直线流动状态中的单列细胞或生物颗粒进行逐个、多参数、快速的定性、定量分析或分选的技术，具有检测速度快、测量指标多、采集数据量大、分析全面、方法灵活等特点。流式细胞术已被众多的研究人员作为优先选择的研究技术。目前，流式细胞术已广泛应用于免疫学、细胞生物学、遗传学、生物化学、肿瘤学、血液学等基础学研究和临床医学领域。
目前，Biolegend公司是世界上主要的流式抗体生产商，Biolegend公司作为后起之秀，以其过硬的质量、更低的价格、丰富的规格、优质的服务及快速的到货期为越来越多的科研工作者所青睐！
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