Takara Clontech Y30035 Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™ Culture System

产品编号  Y30035 | Takara Clontech

Cellartis Definitive Endoderm Differentiation Kit

• The Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System contains a complete media set and a ready-to-use coating for the differentiation of human induced pluripotent stem (hiPS) cells into definitive endoderm (DE) cells in monolayer culture. The kit includes the Cellartis DEF-CS 100 Culture System for the maintenance of undifferentiated hiPS cells prior to DE cell differentiation. The DE cells obtained with this differentiation kit can be used for further differentiation into cells of endodermal origin, such as hepatocytes and pancreatic endoderm cells. For further differentiation of DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050); alternately, start directly from iPS cells using the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).
• DE cell differentiation from two separate hiPS cell lines using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium shows the expected temporal mRNA expression profiles for endodermal markers CXCR4, FOXA2, SOX17, and SOX7; mesoendodermal markerBrachyury; and pluripotency marker OCT4. See the Images & Data tab, below.

产品描述

Features

Efficient differentiation—generates >80% SOX17-positive cells

Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines

Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells

Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation

Components

Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System (Cat. No. Y30035) contains:

Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030; not sold separately)




Definitive Endoderm Diff Coating	10 ml
Definitive Endoderm Diff Day 0	18 ml
Definitive Endoderm Diff Day 1	18 ml
Definitive Endoderm Diff Day 2	18 ml
Definitive Endoderm Diff Day 3	25 ml
Definitive Endoderm Diff Day 4	47 ml
Definitive Endoderm Diff Day 6	47 ml




Cellartis DEF-CS 100 Culture System (Cat. No. Y30020; not sold separately. A larger 500-ml version of this product is sold as Cat. No. Y30010.)




DEF-CS Basal Medium	100 ml
DEF-CS COAT-1 (for 100 ml)	800 µl
DEF-CS GF-1 (for 100 ml)	300 µl
DEF-CS GF-2 (for 100 ml)	100 µl
DEF-CS GF-3 (for 100 ml)	40 µl




Additional Product Information

• Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.



公司简介

美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。

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