Takara Clontech Y40030 GS2-M®

产品编号  Y40030 | Takara Clontech

Maintenance of Germline-Competent Mouse and Human ES and iPS Cells: GS2-M Medium

• GS2‐M is a completely defined, serum‐free cell culture media formulation for the generation and long-term maintenance of human and mouse embryonic stem (ES) and induced pluripotent stem (iPS) cell lines. The medium contains two selective small molecule inhibitors that block differentiation‐inducing signals and promote cell survival.
• GS2-M medium can be used in combination with the self-renewal cytokine leukemia inhibitory factor (LIF) to convert partial or pre‐iPS cells from mouse into fully pluripotent iPS cells via upregulation of Nanog (1, 2).

产品描述

Features

Completely defined, serum-free formulation

Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK and maintain ES and iPS cells in a pluripotent state

Allows long-term maintenance of germline-competent mouse and human ES and iPS cells

Applications

Conversion of partial or pre-iPS cells from mouse into fully pluripotent stem cells*

Conversion of human pre-ES or iPS cells into naïve stem cells*

Maintenance of pure ES and iPS cells in a pluripotent state

• *In the presence of Forskolin and/or LIF

Components

100 ml medium

100 µl 2i inhibitor supplement

References

Silva, J. et al. (2008) Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition. PLoS Biol. 6:e253.

Silva, J. et al. (2009) Nanog is the Gateway to the Pluripotent Ground State. Cell138(4):722–737.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.

公司简介

美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。

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