Takara Clontech Y40020 GS1-R®

产品编号  Y40020 | Takara Clontech

Maintenance, Propagation, and Manipulation of Rat ES and EG Cells: GS1-R Medium

• GS1‐R is a completely defined, serum‐free cell culture medium that is used for the derivation, maintenance, and propagation of rat embryonic stem (ES) and embryonic germ (EG) cells. The medium contains selective small molecule inhibitors that block differentiation-inducing signals, maintaining cells in the ‘ground state’ of self-renewal and promoting cell survival and growth (1–3). Rat ES cells cultured in GS1‐R medium are germ-line competent and can be used to generate transgenic knock‐out rats using either classic homologous recombination or nuclease-based gene editing techniques.

产品描述

Features

Completely defined formulation; serum- and feeder-free

Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK

Culture in this medium allows rat ES cells to be efficiently derived, propagated, and genetically manipulated

Applications

Derivation and long‐term maintenance of germline-competent rat ES and EG cells

Components

100 ml medium

100 µl 2i inhibitor supplement

References

Li, P. et al. (2008) Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts. Cell 135(7):1299–310.

Buehr, M. et al. (2008) Capture of Authentic Embryonic Stem Cells from Rat Blastocysts.Cell 135(7):1287–98.

Leitch, H. et al. (2013) Naive pluripotency is associated with global DNA hypomethylation.Nat. Struct. Mol. Biol. 20:311–316.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.

公司简介

美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。

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