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Takara Clontech  Y30051    Cellartis® Hepatocyte Maintenance Medium

产品编号  Y30051  | Takara Clontech

Cellartis iPS Cell to Hepatocyte Differentiation System

Hepatocytes derived from human induced pluripotent stem (hiPS) cells using the Cellartis iPS Cell to Hepatocyte Differentiation System are an alternative to primary hepatocytes as they exhibit sufficient expression levels of drug-metabolizing enzymes and transporters and demonstrate stable functionality over time in culture. In addition, hiPS cell-derived hepatocytes can provide an accurate reflection of the metabolic diversity observed in the human population. The Cellartis iPS Cell to Hepatocyte Differentiation System provides a complete solution for generating functional, hiPS cell-derived hepatocytes within three weeks.

The process of generating hiPS cell-derived hepatocytes begins with the directed differentiation of hiPS cells into Definitive Endoderm (DE) cells that are then differentiated further into hepatocytes. The complete system provides media and ready-to-use coatings for each step of the iPS-cell-to-hepatocyte differentiation protocol. Starting with approximately 3 x 106undifferentiated hiPS cells, this system yields 5 x 106hepatocytes, equivalent to a confluent monolayer of 50 cm2. This do-it-yourself system offers a solution for the consistent production of assay-ready cells from patient-derived cells or from Cellartis brand iPS cell lines—enabling highly reproducible results.

Hepatocytes obtained with the Cellartis iPS Cell to Hepatocyte Differentiation System express major hepatic markers and show relevant CYP enzyme activities based on the genetic background of the original hiPS cells from which they were derived. In addition, these freshly differentiated hepatocytes are functionally stable for a longer timeframe compared to cryopreserved primary hepatocytes, enabling prolonged experiments such as long-term toxicity assays or viral infection studies. Cellartis Hepatocyte Maintenance Medium (Cat. No. Y30051) is recommended for long-term maintenance of hiPS cell-derived hepatocytes.

产品描述

Features

ØHighly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so no need to optimize for your lines.

ØIdeal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds.

ØCustomized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models.

ØReady for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

ØExtended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

Components

ØCellartis DEF-CS 100 Culture System (Y30020, not sold separately)

ØCellartis Definitive Endoderm Differentiation Kit (Y30030, not sold separately)

ØCellartis Hepatocyte Differentiation Kit (Y30050)

References

1 Asplund, A., et al.(2015) Stem Cell Rev. and Rep.Advance online publication. doi: 10.1007/s12015-015-9621-9

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

公司简介

美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美第一家，也是亚裔创办的最大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。

美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

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