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BD Pharmingen™ 562496 Human Definitive and Pancreatic Endoderm Analysis Kit
产品编号 562496 |BD Pharmingen™
(RUO)
产品描述
Brand
BD Stemflow™
Reactivity
Human (QC Testing)
Application
Intracellular staining (flow cytometry) (Routinely Tested)
Regulatory Status
产品详情
DESCRIPTION
- The BD Stemflow™ Human Definitive and Pancreatic Endoderm Analysis Kit contains a combination of mouse monoclonal antibody conjugates for the analysis of definitive and pancreatic endodermal differentiation cultures. The antibody specificities that are provided in this kit and the cell populations they can identify are listed in the table below. The antibody components of this kit can be used individually or in combinations of up to four. Additional antibody formats that can enable more complex panels are available at www.bdbiosciences.com. The kit also includes BD Cytofix™ Fixation Buffer and BD Phosflow™ Perm Buffer III.
- Kit Components
- Component Description Size Vol. Per Test Storage Buffer
- 51-9008006 Alexa Fluor® 488 Mouse Anti-Human Pax-6 25 Test 5 µl Aqueous buffered solution containing
- BSA and ≤0.09% sodium azide
- 51-9008007 PE Mouse Anti-PDX-1 25 Test 5 µl Aqueous buffered solution containing
- BSA and ≤0.09% sodium azide
- 51-9008008 PE Mouse Anti-Human FoxA2 25 Test 5 µl Aqueous buffered solution containing
- BSA and ≤0.09% sodium azide
- 51-9007227 PerCP-Cy™5.5 Mouse Anti-Sox2 25 Test 5 µl Aqueous buffered solution containing
- BSA and ≤0.09% sodium azide
- 51-9008009 PerCP-Cy™5.5 Mouse Anti-Human Sox17 25 Test 5 µl Aqueous buffered solution containing
- BSA and ≤0.09% sodium azide
- 51-9008010 Alexa Fluor® 647 Mouse Anti-Human Nanog 25 Test 5 µl Aqueous buffered solution containing
- BSA and ≤0.09% sodium azide
- 51-9006607 Perm Buffer III 50 ml
- 51-9006276 Fixation Buffer 50 ml
- Specificity Clone Cell Population Identified
- Nanog N31-335 Pluripotent stem cells
- Sox2 O30-678 Pluripotent stem cells, ectodermal contaminants
- Sox17 P7-969 Definite endoderm
- FoxA2 N17-280 Definitive endodermPax6 O30-1330 Pancreatic endoderm, ectodermal contaminants
- Pdx1 658A5 Pancreatic endoderm
SUGGESTED COMPANION PRODUCTS
Cat No. | Description | Size |
100 mL | ||
500 mL | ||
6 | ||
50 Tests | ||
0.1 mg | ||
100 Tests | ||
100 Tests | ||
0.1 mg | ||
50 Tests | ||
50 µg | ||
0.1 mg | ||
0.1 mg | ||
100 Tests |
RESOURCES & TOOLS
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
PRODUCT NOTICES
Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
This PerCP-conjugated product is sold under license to the following patent: US Patent No. 4,876,190.
Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
公司简介
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BD公司的业务共分为三大类: BD医疗, BD生物科学, BD诊断
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