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BD Pharmingen™  561526  Human Neural Lineage Analysis Kit  (RUO)

产品编号  561526|BD Pharmingen™

Human Neural Lineage Analysis Kit  (RUO)

产品描述

Brand

BD Stemflow™

Reactivity

Human (QC Testing)

Application

Flow cytometry (Routinely Tested)

Regulatory Status

RUO

Sizes

25 Tests

Catalog No.

561526

List Price

¥ 10,983.00

Your Price

Unit

EA (1 Each)

产品详情

DESCRIPTION

The BD Stemflow™ Human Neural Lineage Analysis Kit contains a combination of mouse monoclonal antibody conjugates for the analysis of neural differentiation cultures. The antibody specificities that are provided in this kit and the neural cell populations they can identify are listed in the table below. The antibody components of this kit can be used individually or in combinations of up to four. All Antibodies in this kit have been verified by western blot and image analysis. Additional antibody formats that can enable more complex panels are available at www.bdbiosciences.com. The kit also includes BD Cytofix™ Fixation Buffer and BD Phosflow™ Perm Buffer III.

Kit Components

Component Description Size Vol. Per Test Storage Buffer

51-9007227   PerCP-Cy™5.5 Mouse Anti-Sox2            25 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤ 0.09% sodium azide

51-9007228   Alexa Fluor® 647 Mouse Anti-GFAP         25 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤ 0.09% sodium azide

51-9007229   PE Mouse Anti-Doublecortin               25 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤ 0.09% sodium azide

51-9007230   Alexa Fluor® 647 Mouse Anti-Nestin         25 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤ 0.09% sodium azide

51-9007231   Alexa Fluor® 488 Mouse Anti-Human Ki-67   25 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤ 0.09% sodium azide

51-9007232   PerCP-Cy™5.5 Mouse Anti-Human Sox1      25 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤ 0.09% sodium azide

51-9007233   FITC Mouse Anti-Human CD44            25 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤ 0.09% sodium azide

51-9006607   Perm Buffer III                     50 ml            

51-9006276   Fixation Buffer                     50 ml

Specificity      Clone         Molecule               Neural cell population identified

CD44         G44-26         H-CAM               Glial cells, astrocytes, astrocyte precursors

Ki-67         B56            Nuclear proliferation marker   All proliferating cell types

Doublecortin      30/Doublecortin   Neuroflilament         Immature post-mitotic neurons

Sox2         O30-678         Transcription factor         Glial cells, embryonic and neural stem cells

Sox1         N23-844         Transcription factor         Glial cells and neural stem cells

GFAP         1B4            Filament               AstrocytesNestin         25/Nestin         Filament               Glial cells, astrocytes, embryonic and neural stem cells

SUGGESTED COMPANION PRODUCTS

RESOURCES & TOOLS

Download SDS

Download Data Sheet

PREPARATION AND STORAGE

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

PRODUCT NOTICES

Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.

Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).

Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).

Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.

The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.

PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.

PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.

This PerCP-conjugated product is sold under license to the following patent: US Patent No. 4,876,190.

Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.

This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.

Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

公司简介

BD是一家全球化的医疗技术公司，通过改善医学发现方法、医疗诊断效果和护理质量以引领世界健康。BD在保护患者和医护人员安全、强化医学研究和临床实验室建设领域居于世界领先行列。公司提供创新的解决方案，以帮助推进细胞学和基因组学研究、加强传染性疾病和癌症的诊断、改善药物管理、促进感染预防、为手术和介入治疗提供工具、优化呼吸治疗，并支持糖尿病管理。BD与全球范围内的机构组织携手，共同应对最具挑战的全球健康问题。公司在逾50个国家拥有分支机构，员工超过45,000人，公司与客户及合作伙伴紧密协作，以帮助改善医疗结果，降低医疗护理成本，提升效率，保护患者和医护人员安全并增加医疗可及性。

BD目前经营产品超过一万余种，是全球知名的医用一次性产品供应商。公司上市的所有产品均遵循同行业最高标准，并通过ISO国际质量认证。

在一百多年的发展进程中，BD以其雄厚的实力相继收购了近20家医疗器械和医疗设备公司，扩大了公司经营规模，丰富了产品线，提升了综合竞争能力，为世界同行所瞩目。

BD公司的业务共分为三大类: BD医疗, BD生物科学, BD诊断

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