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BD Pharmingen™ 562271 Human Neural Cell Sorting Kit(RUO)
产品编号 562271|BD Pharmingen™
Human Neural Cell Sorting Kit (RUO)
产品描述
Brand
BD Stemflow™
Reactivity
Human (Reactivity Confirmed in Development)
Regulatory Status
RUO
Sizes
10 Tests
Catalog No.
562271
List Price
¥ 10,983.00
Your Price
Unit
EA (1 Each)
产品详情
DESCRIPTION
The BD Stemflow™ Neural Cell Isolation Kit was designed to allow the isolation of neural stem cells (NSCs) derived from human pluripotent stem cells or the isolation of neurons and glial cells from differentiated NSCs. Following a variety of established neural induction protocols, NSCs are sorted from the heterogeneous cell culture utilizing the included antibody conjugates to five cell surface markers. This method greatly reduces the amount of variability between NSC isolations as opposed to traditional methods like manual isolation. In addition, four of the five included conjugates can be used to isolate neurons from glia from differentiated NSCs.
Kit Components
Component Description Size Vol. Per Test Storage Buffer
51-9007757 PE Mouse Anti-Human CD24 20 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007758 PerCP-Cy™5.5 Mouse Anti-Human CD271 20 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007759 PerCP-Cy™5.5 Mouse Anti-Human CD44 20 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007760 PE-Cy™7 Mouse Anti-Human CD15 20 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007761 APC Mouse Anti-Human CD184 20 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007762 PE Mouse IgG1, k Isotype Control 10 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007763 PerCP-Cy™5.5 Mouse IgG1, k 10 Test 5 µl Aqueous buffered solution containing
Isotype Control for 51-007758 BSA and <0.09% sodium azide
51-9007764 PerCP-Cy™5.5 Mouse IgG1, k 10 Test 5 µl Aqueous buffered solution containing
Isotype Control for 51-9007759 BSA and <0.09% sodium azide
51-9007765 PE-Cy™7 Mouse IgM, k Isotype Control 10 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007766 APC Mouse IgG1, k Isotype Control 10 Test 5 µl Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007767 Negative Control CompBead Plus 1.5 ml 1 drop Aqueous buffered solution containing
BSA and <0.09% sodium azide
51-9007768 Anti-Mouse Ig, k CompBead Plus 1.5 ml 1 drop Aqueous buffered solution containing
BSA and <0.09% sodium azide
Specificity Clone Molecule NSC Phenotype Neuron Phenotype Glial Phenotype
CD15 HI98 X-hapten, SSEA-1 -/+ Low NA
CD24 ML5 Heat Stable Antigen + + NA
CD44 G44-26 H-CAM - - +
CD184 12G5 CXCR4, Fusin + - +
CD271 C40-1457 NGF-Receptor - NA NA
SUGGESTED COMPANION PRODUCTS
Cat No. | Description | Size |
560497 | Anti-Mouse Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set RUO | 6 |
561527 | Accutase™ Cell Detachment Solution RUO | 100 mL |
RESOURCES & TOOLS
Download TDS
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
PRODUCT NOTICES
Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
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BD公司的业务共分为三大类: BD医疗, BD生物科学, BD诊断
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