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BD Pharmingen™  562245  Human MSC Analysis Kit  (RUO)

产品编号  562245|BD Pharmingen™

Human MSC Analysis Kit  (RUO)

产品描述

Brand

BD Stemflow™

Reactivity

Human (Tested in Development)

Application

Flow cytometry (Tested During Development)

Regulatory Status

RUO

Sizes

50 Tests

Catalog No.

562245

List Price

¥ 21,122.00

Your Price

Unit

EA (1 Each)

产品详情

DESCRIPTION

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues. MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes. MSCs also have immunomodulatory effects in vivo and in vitro. In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow. Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α , and HLA-DR. MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271. In this kit we include the panel that was proposed by the ISCT. In addition, we designed this kit to be modular so that additional markers could be “dropped-in”. Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5 CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PE CD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibody conjugates. Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocol to analyze multiple samples. Individual positive markers are included for compensation set-up.

Kit Components

Component   Description                        Size      Vol. Per Test   Storage Buffer

51-9007661   PE hMSC Negative Cocktail               50 Test      20 µl   Aqueous buffered solution containing

                                                     BSA and ≤0.09% sodium azide

51-9007662   PE hMSC Isotype Control Negative Cocktail   50 Test      20 µl   Aqueous buffered solution containing

                                                     BSA and ≤0.09% sodium azide

51-9007663   hMSC Positive Cocktail                  50 Test      20 µl   Aqueous buffered solution containing

                                                     BSA and ≤0.09% sodium azide

51-9007664   hMSC Isotype Control Positive Cocktail      50 Test      20 µl   Aqueous buffered solution containing

                                                     BSA and ≤0.09% sodium azide

51-9007657   FITC Mouse Anti-Human CD90            50 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤0.09% sodium azide

51-9007656   PE Mouse Anti-Human CD44            100 Test      5 µl      Aqueous buffered solution containing BSA,

                                                     protein stabilizer, and ≤0.09% sodium azide

51-9007655   PE Mouse IgG2b, k Isotype Control         50 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤0.09% sodium azide

51-9007649   APC Mouse Anti-Human CD73            50 Test      5 µl      Aqueous buffered solution containing

                                                     BSA and ≤0.09% sodium azide

51-9007648   PerCP-Cy™5.5 Mouse Anti-Human CD105      50 Test      5 µl      Aqueous buffered solution containing

         (Endoglin)                                       BSA and ≤0.09% sodium azide

Description of Kit Components

Vial                              Contents                     Purpose  

hMSC Positive Cocktail                  CD90 FITC (Clone: 5E10)         Cocktail to positively identify hMSCs

                             CD105 PerCP-Cy5.5 (Clone: 266)

                             CD73 APC (Clone: AD2)

hMSC Positive Isotype Control Cocktail      mIgG1, κ FITC (Clone: X40)         Corresponding Isotype Control for hMSC Positive                              mIgG1, κ PerCP-Cy5.5 (Clone: X40)   Cocktail

                             mIgG1, κ APC (Clone: X40)

PE hMSC Negative Cocktail               CD34 PE (Clone:581)            Cocktail to identify potential contaminants

                             CD11b PE (Clone: ICRF44)

                             CD19 PE (Clone: HIB19)

                             CD45 PE (Clone: HI30)

                             HLA-DR PE (Clone: G46-6)

PE hMSC Negative Isotype Control Cocktail   mIgG1, κ PE (Clone: X40)         Corresponding isotype control for PE hMSC

                             mIgG2a, κ PE (Clone:G155-178)      Negative Cocktail

FITC Mouse Anti-human CD90            CD90 FITC (Clone: 5E10)         Compensation control

PE Mouse Anti-Human CD44            CD44 PE    (Clone: G44-26)         Compensation control/MSC positive drop-in

PerCP-Cy™5.5 Mouse Anti-Human CD105      CD105 PerCP-Cy™5.5 (Clone: 266)   Compensation control

APC Mouse Anti-Human CD73            CD73 APC (Clone:AD2)         Compensation control

PE Mouse IgG2b, κ Isotype Control         mIgG2b, κ (Clone: 27-35)         Corresponding Isotype Control for PE Mouse

                                                     Anti-Human CD44, when used as a drop in

SUGGESTED COMPANION PRODUCTS

RESOURCES & TOOLS

Download Data Sheet

PREPARATION AND STORAGE

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

PRODUCT NOTICES

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.

This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.

PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.

PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.

Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.

This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.

Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

公司简介

BD是一家全球化的医疗技术公司，通过改善医学发现方法、医疗诊断效果和护理质量以引领世界健康。BD在保护患者和医护人员安全、强化医学研究和临床实验室建设领域居于世界领先行列。公司提供创新的解决方案，以帮助推进细胞学和基因组学研究、加强传染性疾病和癌症的诊断、改善药物管理、促进感染预防、为手术和介入治疗提供工具、优化呼吸治疗，并支持糖尿病管理。BD与全球范围内的机构组织携手，共同应对最具挑战的全球健康问题。公司在逾50个国家拥有分支机构，员工超过45,000人，公司与客户及合作伙伴紧密协作，以帮助改善医疗结果，降低医疗护理成本，提升效率，保护患者和医护人员安全并增加医疗可及性。

BD目前经营产品超过一万余种，是全球知名的医用一次性产品供应商。公司上市的所有产品均遵循同行业最高标准，并通过ISO国际质量认证。

在一百多年的发展进程中，BD以其雄厚的实力相继收购了近20家医疗器械和医疗设备公司，扩大了公司经营规模，丰富了产品线，提升了综合竞争能力，为世界同行所瞩目。

BD公司的业务共分为三大类: BD医疗, BD生物科学, BD诊断

BD医疗——下设医疗系统,糖尿病护理,制药系统三个部门。医疗系统的产品涉及输液治疗、安全注射、麻醉系统等三个领域。糖尿病护理业务部门的产品主要有胰岛素注射器、笔用针头及糖尿病护理产品。制药系统生产供制药企业使用的预充式注射器等。

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