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BD Pharmingen™ 561209 APC Mouse Anti-Human CD34
产品编号 561209 |BD Pharmingen™
Clone 563 (RUO)
产品描述
Brand
BD Pharmingen™
Alternative Name
gp 105-120
Vol. Per Test
5 µl
Isotype
Mouse IgG1, κ
Reactivity
Rhesus, Cynomolgus, Baboon (QC Testing)
Application
Flow cytometry (Routinely Tested)
Workshop No.
V MA030
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
产品详情
DESCRIPTION
- The 563 monoclonal antibody specifically binds to CD34. CD34 is a single-chain 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 563 belongs to the class III epitope; it is resistant to neuraminidase, chymopapain and glycoprotease. This antibody is able to block the binding of other CD34 mAbs like 581.
- Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
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FORMAT
Format
APC
Excitation Source
Red 633 nm
Excitation Max
650 nm
Emission Max
660 nm
Other Formats →
SUGGESTED COMPANION PRODUCTS
Cat No. | Description | Size | |
500 mL |
100 Tests |
RESOURCES & TOOLS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
公司简介
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