R&D  CDK006 CellXVivo Human Treg Cell Differentiation Kit

产品编号 CDK006 | rndsystems

CellXVivo Human Treg Cell Differentiation Kit

产品介绍

Product Details

Kit Summary

For the differentiation of Human Regulatory T (Treg) Cells from a preparation of naïve CD4⁺ T cells.

Key Benefits

Contains high quality bioactive proteins

Yields a highly enriched population of FoxP3⁺CD25⁺ Treg cells

Provides optimized reagents for inducing Tregs from CD4⁺ T cells

Involves validated and straightforward procedures

Does not require specialized instrumentation

Why Differentiate Treg Cells In Vitro?

• Forkhead Box P3 (FoxP3)⁺ regulatory T (Treg) cells are a suppressive subset of CD4⁺ T cells that function to antagonize immune responses. Treg cells have the capacity to prevent potentially damaging autoimmune and protective immune responses, so the number of Treg cells is a crucial determinant of the regulatory burden on the immune system. In vitro differentiation of Treg cells from the larger naïve CD4⁺ T cell population provides increased numbers of Treg cells to facilitate downstream research.

Reagents Provided

• This kit contains the following optimized proteins and reagents to drive efficient differentiation of naïve CD4⁺ T cells into FoxP3⁺CD25⁺ Treg cells.

Mouse Anti-Human CD3 Antibody

Human Treg Reagent 1

Human Treg Reagent 2

Human Treg Reagent 3

Human Treg Reagent 4

Reconstitution Buffer 1

Reconstitution Buffer 2

Wash Buffer (20X)

• Provides sufficient reagents for the differentiation of two 24-well plates.

Stability and Storage

• Store the unopened kit at 2-8 °C. After opening the kit, the Mouse Anti-Human CD3 Antibody and Human Treg Reagents 1-4 may be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 3 months at 2-8 °C. Storage conditions are valid if used within the expiration date of the kit.

Data Examples

Differentiation of Human CD4⁺ T Cells into Treg Cells Confirmed by FoxP3 and CD25 Expression. Human peripheral blood naïve CD4⁺ T cells were incubated with reagents included in the Human Treg Cell Differentiation Kit for 5 days. Cells were fixed, permeabilized, and stained using the FlowX™ Human Regulatory T Cell Kit (Catalog # FMC021). Cells were imaged using flow cytometry. Quadrants were set based on isotope-stained samples.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Related Research Areas

T Cell Kits

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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