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R&D CDK002 CellXVivo Human Th2 Cell Differentiation Kit
产品编号 CDK002| rndsystems
CellXVivo Human Th2 Cell Differentiation Kit
产品介绍
Kit Summary
For the differentiation of Th2 cells from a preparation of naïve CD4+ T cells.
Key Benefits
Contains high quality bioactive proteins
Yields a highly enriched population of Th2 polarized T cells
Provides optimized reagents needed to induce Th2 differentiation
Involves validated and straightforward procedures
Does not require specialized instrumentation
Why Differentiate Th2 Cells In Vitro?
T helper type 2 (Th2) cells are a lineage of CD4+ effector T cells that provide host protection against intestinal helminths and extracellular bacteria in addition to support for B cell-dependent humoral responses. Pathological Th2 cell activity is a hallmark of allergic inflammation and asthma. Differentiation of CD4+ effector cells into the Th2 lineage is promoted by cytokines such as IL-4 in combination with either IL-2, IL-7, or TSLP. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL17E/IL-25. The CellXVivo™ Human Th2 Cell Differentiation Kit contains all necessary components to differentiate human naïve CD4+ T cells into Th2 polarized cells.
Reagents Provided
This kit contains the following optimized proteins and reagents to drive efficient differentiation of naïve CD4+ T cells into Th2 polarized cells.
Mouse Anti-Human CD3 Antibody
Human Th2 Differentiation Supplements
Reconstitution Buffers
Wash Buffer (20X)
Provides sufficient reagents for the differentiation of one 24-well plate or 125 tests in a 96-well format.
Stability and Storage
Store the unopened kit at 2 to 8°C. After opening the kit, Mouse Anti-Human CD3 Antibody and Human Th2 Reagents 1-4 may be stored at 2-8°C under sterile conditions for up to 30 days or at -20°C to -70°C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 12 months at 2 to 8°C. Do not use beyond the expiration date of the kit.
Data Examples
Intracellular Cytokine Staining of Differentiated Human Th2 Cells. Flow cytometry data showing human peripheral blood naïve CD4+ T cells without (A, C) and with (B, D) a 13 day differentiation using reagents included in the Human Th2 Cell Differentiation Kit. On day 13 of differentiation, the cells were re-stimulated with mitogens and stained with a PerCP-conjugated Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog #IC3171C), an APC-conjugated Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # IC285A), and a PE-conjugated Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # IC204P). Quadrants were set based on isotype control-stained samples.
Detection of IL-5, IFN-gamma, and IL-17 in Differentiated Human Th2 Cells. Human peripheral blood na ve CD4+ T cells were differentiated for 13 days using reagents included in the Human Th2 Cell Differentiation Kit. On day 13, cell culture supernatant was collected and cytokine secretion was determined using the Human IL-5 Quantikine ELISA Kit (D5000B), the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50), and the Human IL-17 Quantikine ELISA Kit (Catalog # D1700). |
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 掳C. Do not use past expiration date.
公司简介
R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。
目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。
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