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R&D CDK001 CellXVivo Human Th1 Cell Differentiation Kit
产品编号 CDK001| rndsystems
CellXVivo Human Th1 Cell Differentiation Kit
产品介绍
Kit Summary
For the differentiation of Th1 cells from a preparation of naïve CD4+ T cells.
Key Benefits
Yields a highly enriched population of Th1 polarized T cells
Provides optimized reagents needed to induce Th1 differentiation
Involves validated and straightforward procedures
Does not require specialized instrumentation
Why Differentiate Th1 Cells In Vitro?
T helper type 1 (Th1) cells are a subset of CD4+ effector T cells that promote cell-mediated immune responses against intracellular viral and bacterial pathogens. Differentiation into the Th1 lineage is promoted by cytokines such as IL-12 and IFN-gamma. Th1 cells are characterized by the secretion of IFN-gamma, IL-10, and TNF-alpha. Although Th1 cells are critical for the clearance of intracellular pathogens, exaggerated Th1 responses are associated with autoimmune diseases including rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, and type 1 diabetes. Th1 polarized cells are present in low abundance in normal human peripheral blood. In vitro differentiation of Th1 cells from the larger naïve CD4+ T cell population provides increased numbers of Th1 cells to facilitate downstream research.
Reagents Provided
This kit contains the following optimized reagents to drive efficient differentiation of naïve CD4+ T cells into Th1 polarized cells.
Mouse Anti-Human CD3 Antibody
Human Th1 Differentiation Supplements
Reconstitution Buffers
Wash Buffer (20X)
Provides sufficient reagents for the differentiation of one 24-well plate or 250 tests in a 96-well format.
Stability and Storage
Store the unopened kit at 2 to 8 °C. After opening the kit, Mouse Anti-Human CD3 Antibody, Human Th1 Reagent 1, and Human Th1 Reagent 2 may be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 12 months at 2 to 8 °C. Do not use beyond the expiration date of the kit.
Data Examples
Verification of Th1 Cell Identity using Flow Cytometry. Human peripheral blood naïve CD4+ T cells without (A, C) and with (B, D) a 5 day differentiation using the reagents included in the Human Th1 Cell Differentiation Kit. (A, B) The cells were stained with an APC-conjugated Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog #IC285A) and a PE-conjugated Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # IC204P). (C, D) The cells were stained with an APC-conjugated Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog #IC285A) and a PerCP-conjugated Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog #IC3171C). Control cultures were used to place the quadrants.
Detection of IFN-gamma, IL-4, and IL-17 in Human Th1 Cells. Human peripheral blood naïve CD4+ T cells were cultured for 5 days using reagents included in the Human Th1 Cell Differentiation Kit. After fixation of cells in the wells, cytokine expression was determined using the Human IFN-gamma Quantikine ELISA Kit (Catalog #DIF50), the Human IL-4 Quantikine ELISA Kit (Catalog #D4050), and the Human IL-17 Quantikine ELISA Kit (Catalog #D1700). |
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
公司简介
R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。
目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。
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