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R&D  CCM019  CryoDefend-Cell Lines (5 x 10 mL)

产品编号 CCM019| rndsystems

CryoDefend-Cell Lines (5 x 10 mL)

For cryopreservation of cell lines

产品介绍

Media for defined, protein-free cryopreservation of cultured cells.

Key Benefits

Reduce experimental variability by using fully defined media

Supports post-thaw cell viability better than conventional freezing media

Optimized for cryopreservation of cultured cells

Suitable for all cell types

Why use fully defined, protein-free media to cryopreserve cells?

Cryopreservation can be used to maintain cells at a low passage number and to reduce the risk of contamination by microbes and other cell types. The viability of the recovered cells depends on cell density, the type of cryoprotectant used, the method of cooling, and the amount of the cryoprotectant that remains in the culture after the cells have been thawed.

R&D Systems offers CryoDefend Cell Lines Media, a defined, protein-free media, which has been optimized for the cryopreservation of cultured cells. This specially formulated media contains a defined serum substitute as well as an optimized concentration of a cryopreservative that increases the recovery and viability of healthy cells compared to conventional freezing media.

Kit Contents

Components

5 x 10 mL vials of CryoDefend Stem Cells Media

Data Examples

Cell Viability Following Cryopreservation in CryoDefend-Cell Lines Media in Comparison to Conventional Cryopreservation Media. A variety of cell lines were frozen in triplicate in either control freezing media (90% culture media/10% DMSO) or CryoDefend-Cell Lines (CryoDefend-CL) media at 1 x 106 cells/cryovial and stored in liquid nitrogen for one week. The cells were then thawed, counted (blue bars), resuspended in standard culture media, and plated into T75 flasks. After 3-5 days in culture, the cells were harvested and counted (red bars). Error bars shown indicate the standard deviation.

Preparation and Storage

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

公司简介

R&D Systems作为全球免疫学和细胞生物学产品的领跑者，1976年创立于美国明尼苏达州。其母公司Bio-Techne（原Techne公司）于1983年在美国NASDAQ上市。

目前，R&D Systems拥有近30,000种产品，95%以上由自己研发生产。其产品包括细胞因子、ELISA试剂盒、生长因子、趋化因子、抗体、Animal-Free™蛋白、Luminex 液相芯片、多因子检测固相芯片、流式细胞分析与细胞筛选、干细胞及细胞培养等；覆盖肿瘤、发育、糖生物、内分泌、免疫、神经学、蛋白酶、信号传导等学科研究领域；且每年有近2,000种新产品不断问世，以满足不同科研工作者的研究需要。

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