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InvivoGen ant-gn-1 G418 (Geneticin)
G418 (Geneticin)
Selection antibiotic: cell culture tested, sterile reagent
G418, also known as G418 sulfate and Geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘ II.
Selection in mammalian cells is usually achieved in three to seven days with concentrations ranging from 400 to 1000 µg/ml. Cells that are dividing are affected sooner than those that are not.
Specifications Product concentration: 100 mg/mlCAS number: 108321-42-2
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.Purity: ≥ 90% (HPLC)Endotoxin level: < 0.5 EU/mgPhysicochemical characterization: pH, appearanceCell-culture tested: potency validated in G418-sensitive and G418-resistant mammalian cell linesNon-cytotoxicity of trace contaminants: absence of long-term effects confirmed in G418-resistant cells
ContentsG418 is supplied as a sterile filtered solution at 100 mg/ml in HEPES buffer. This product is available in two pack sizes: - ant-gn-1: 10 x 1 ml (1 g)- ant-gn-5: 1 x 50 ml (5 g)
G418 is shipped at room temperature. Upon receipt it should be stored at 4°C.
产品描述
Selective Antibiotics
InvivoGen is a leader in the production of selection antibiotics. Our state-of-the-art facilities allow us to produce large quantities of high quality, endotoxin-free antibiotics with purity levels exceeding 95%. As we manufacture our products, we are able to offer the best prices on the market.
Ready-to-use Cell Culture Tested Solutions - No weighing needed - Our antibiotics are available in solution filtered to sterility for customer convenience and validated for cell culture usage.
An Excellent Choice of Antibiotics for Selection in Both Mammalian cells and E. coli - Matches up to the antibiotic resistance genes carried by InvivoGen plasmids, built for selection in both mammalian and bacteria cells.
窗体顶端 窗体底端 | Selective antibiotic for the bsr or BSD genes | 1 g 100 mg (10 x 1 ml) 500 mg (50 ml bottle) 500 mg (50 x 1 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the Sh ble gene | 1 g (powder) 1g (10 x 1 ml) 5g (50 x 1 ml) 5 g (50 ml bottle) 5 g (powder) |
窗体顶端 窗体底端 | Selective antibiotic for the pac gene | 500 mg (50 x 1 ml) 500 mg (1 x 50 ml) 100 mg (10 x 1 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the neo gene | 1 g (10 x 1ml) 5 g (1 x 50 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the hph gene - Hygromycin B High purity | 1 g (10 x 1 ml) 5 g (1 x 50 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the Sh ble gene | 1 g 100 mg (5 x 1 ml) 500 mg (25 x 1 ml) 500 mg (25 ml) 250 mg 500 mg |
其他产品线
1-Cell Culture and Transfection Reagents:
Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;
2-Mammalian Expression Vectors:
A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;
3-Reporter Cells:
A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;
4-PRR Ligands:
An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;
5-Inhibitors:
A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;
6-Antibodies and Recombinant Proteins:
A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;
7-Vaccination:
Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.
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