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InvivoGen ant-bl-1 Blasticidin
Blasticidin
Selection antibiotic: cell culture tested, sterile reagent
Blasticidin is an efficient selective antibiotic that acts on both eukaryotic and prokaryotic cells. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.
Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase.
Cell death induced by blasticidin is rapid and cell lines carrying a blasticidin resistance gene can be obtained in less than a week. Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml.
Specifications
Product concentration: 10 mg/mlQuality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.Purity: ≥ 95% (HPLC)Endotoxin level: < 1 EU/mgPhysicochemical characterization: pH, appearanceCell-culture tested: potency validated in blasticidin-sensitive and blasticidin-resistant mammalian cell linesNon-cytotoxicity of trace contaminants: absence of long-term effects confirmed in blasticidin-resistant cells
Contents
Blasticidin hydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer. This product is available in three pack sizes:- ant-bl-1: 10 x 1 ml (100 mg)- ant-bl-5: 50 x 1 ml (500 mg)- ant-bl-5b: 1 x 50ml (500 mg)
Blasticidin hydrochloride is also supplied as a powder:- ant-bl-10p: 1 g (powder)
Blasticidin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.
产品描述
Selective Antibiotics
InvivoGen is a leader in the production of selection antibiotics. Our state-of-the-art facilities allow us to produce large quantities of high quality, endotoxin-free antibiotics with purity levels exceeding 95%. As we manufacture our products, we are able to offer the best prices on the market.
Ready-to-use Cell Culture Tested Solutions - No weighing needed - Our antibiotics are available in solution filtered to sterility for customer convenience and validated for cell culture usage.
An Excellent Choice of Antibiotics for Selection in Both Mammalian cells and E. coli - Matches up to the antibiotic resistance genes carried by InvivoGen plasmids, built for selection in both mammalian and bacteria cells.
窗体顶端 窗体底端 | Selective antibiotic for the bsr or BSD genes | 1 g 100 mg (10 x 1 ml) 500 mg (50 ml bottle) 500 mg (50 x 1 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the Sh ble gene | 1 g (powder) 1g (10 x 1 ml) 5g (50 x 1 ml) 5 g (50 ml bottle) 5 g (powder) |
窗体顶端 窗体底端 | Selective antibiotic for the pac gene | 500 mg (50 x 1 ml) 500 mg (1 x 50 ml) 100 mg (10 x 1 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the neo gene | 1 g (10 x 1ml) 5 g (1 x 50 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the hph gene - Hygromycin B High purity | 1 g (10 x 1 ml) 5 g (1 x 50 ml) |
窗体顶端 窗体底端 | Selective antibiotic for the Sh ble gene | 1 g 100 mg (5 x 1 ml) 500 mg (25 x 1 ml) 500 mg (25 ml) 250 mg 500 mg |
其他产品线
1-Cell Culture and Transfection Reagents:
Powerful reagents to prevent microbial contamination in cell culture, plus a wide variety of selection antibiotics and reporter (secreted embryonic alkaline phosphatase (SEAP) or Lucia luciferase) detection reagents, and a transfection reagent;
2-Mammalian Expression Vectors:
A broad array of cloning vectors, that include plasmidic and lentiviral vectors, and CpG-free plasmids, a choice of inducible reporter plasmids, plus an expanding library of human and murine genes, and a wide variety of cellular promoters;
3-Reporter Cells:
A growing collection of functionally validated and mycoplasma-free human and murine cell lines, such as HEK293-, Jurkat-, RAW 264.7- and THP-1-derived cells, that report on one or more pattern recognition receptor (PRR), cytokine, or transcription factor activities using SEAP and/or Lucia luciferase, plus a recently launched line of knockout cells that lack major innate immune sensors or signaling proteins;
4-PRR Ligands:
An expanding collection of agonists and control ligands for all the major PRRs, such as Toll-like receptors (TLRs), NOD-like receptors (NLRs) and cytosolic DNA sensors (CDSs), including ligands of natural and synthetic origin, all screened and validated in-house;
5-Inhibitors:
A wide selection of functionally validated compounds to directly or indirectly block the principal immune (TLR, NLR, CDS) pathways and related signaling pathways;
6-Antibodies and Recombinant Proteins:
A large variety of biologically validated monoclonal and polyclonal antibodies against major human and murine innate immune targets, as well as isotype families of clinically relevant antibodies, plus a selection of recombinant cytokines;
7-Vaccination:
Pre-clinical grade vaccine adjuvants that comprise alum, emulsions, such as CFA and saponin, and PRR ligands, plus various ovalbumin-derived products.
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