Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

￥ 2575.17

产品详情

产品参数

产品评论

Sigma-Aldrich D6421 Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

产品编号 D6421 | SIGMA

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

产品名称

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Reconstitution

Supplement with 0.365 g/L L-glutamine.

Application

DMEM:F12 is a 50:50 mixture of DMEM and Ham’s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Suitable for growth of wide range of cell types in low serum conditions.Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays.^[4]^[1]^[5]^[6] It is also used in bone marrow-derived macrophages culture.^[3]

General description

A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.^[2]

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

性质

Related Categories	Classical Media, Core Bioreagents, Culture,DMEM/Nutrient Mixture F-12 Ham, Dulbecco’s Media, General Stem Cell Biology, Life Science Reagents for Cell Culture, Media, Research Essentials, Stem Cell Expansion, Stem Cell Isolation, 干细胞生物学,细胞培养, 细胞生物学, 经典培养基和盐 Less...
Quality Level	PREMIUM
sterility	sterile-filtered
form	liquid
composition	glucose, 3.15 g/L
	Glutamine: no
	HEPES, 15 mM
	phenol Red: yes
	Sodium bicarbonate (NaHCO3): yes
	Sodium Pyruvate, 0.055 g/L
impurities	endotoxin, tested
suitability	suitable for cell culture
storage temp.	2-8°C