Roche 04693132001  cOmplete™, EDTA-free Protease Inhibitor Cocktail

公司简介

罗氏总部位于瑞士巴塞尔，在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一。作为全球最大的生物技术公司之一，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统领域拥有切实有效的差异化药品；同时罗氏也是体外诊断和基于组织的肿瘤诊断的市场领导者，以及糖尿病管理领域的先驱者。罗氏倡导的“个性化医疗”方案旨在提供创新的药品和诊断工具，从而切实改善人类的健康水准、生活质量以及患者的生存期。

在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。

产品介绍

Features and Benefits

• Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.• Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.• Complete protection: Instantly protect your proteins against a broad range of proteases.• Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.• Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

General description

• cOmplete, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA - no other chelating agents, e.g. EGTA as well - leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).Due to the optimized composition of the tablets, they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.cOmplete EDTA-free Tablets are identical to cOmplete Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.

Other Notes

• 仅用于生命科学研究。不可用于诊断。

包装

• 20 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters.

Preparation Note

• Working concentration: 1 tablet per 50 ml extraction solutionWorking solution: HandlingCarefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.Preparation of Working SolutionOne cOmplete EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 50 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 25 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared.Preparation of Stock solution (25 X conc.)Dissolve one cOmplete EDTA-free tablet in 2 ml dist. water or in 2 ml 100 mM phosphate buffer, pH 7.0.Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

Quality

• Performance-tested with pancreas extract.

Reconstitution

• Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; an be used in thiol-containing solutions at 15 to 25 °C.

Specificity

• Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.

Physical form

• Tablets in foil blister packs

Application

• cOmplete^™, EDTA-free, efficiently inhibits serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.^[2][1][4][3] Metallo- and aspartic proteases are not inhibited.cOmplete, EDTA-free Tablets, are used for the inhibition of proteolytic activity in large volumes (up to 50ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, EDTA-free is preferentially used in the isolation process of Poly-His-tagged fusion proteins.If it is necessary to inhibit proteolytic activity in a smaller volume (up to 10ml), we recommend to use cOmplete, Mini, EDTA-free.One cOmplete, EDTA-free tablet was added per 50 ml incubation solution. Proteolytic activity was determined with the Roche Universal Protease Substrate (casein, resorufin-labeled). When extractions or single-step isolations are necessary in the acid pH range, simply include pepstatin along with cOmplete, EDTA-free tablets to ensure aspartic (acid) protease inhibition. All experiments were performed at +15 to +25°C.

法律信息

• cOmplete is a trademark of Roche

性质




Related Categories	Enzyme Inhibitors, Protease Inhibitors, 生化试剂, 蛋白酶抑制剂Cocktails, 酶、抑制剂和底物
form	tablet
packaging	pkg of 20 tablets (individually packed in foil blister packs)
mfr. no.	Roche
solubility	aqueous buffer: soluble
absorption	A/280 nm 0.08
shipped in	wet ice







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