Roche  11093274910  Anti-Digoxigenin-AP, Fab fragments

公司简介

罗氏总部位于瑞士巴塞尔，在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一。作为全球最大的生物技术公司之一，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统领域拥有切实有效的差异化药品；同时罗氏也是体外诊断和基于组织的肿瘤诊断的市场领导者，以及糖尿病管理领域的先驱者。罗氏倡导的“个性化医疗”方案旨在提供创新的药品和诊断工具，从而切实改善人类的健康水准、生活质量以及患者的生存期。

在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。

产品介绍

Analysis Note

• • Cross reactivity to digitoxin and digitoxigenin: <1 %• No cross reactivity with other human estrogen or androgen steroids, e.g.estradiol or testosterone• Cross reactivity with digoxin: not known• Conjugate does not bind to itself at all• Normally one molecule of the conjugate binds to one molecule digoxigenin, although ther are two possible binding sites for digoxigenin• Nonspecific binding to RNA is not expected

General description

• Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to alkaline phosphatase.

Other Notes

• 仅用于生命科学研究。不可用于诊断。

Preparation Note

• After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: • Dot blot: 150 mU/ml• ELISA: 150 to 300 mU/ml• Immunohistocytochemistry: 250 to 500 mU/ml• In situ hybridization: 1.5 to 7.5 U/ml• Southern blot: 150 mU/ml• Western blot: 250 to 500 mU/mlWorking solution: Northern blot, Southern blot100 mM Maleic acid, 150 mM NaCl, pH 7.5.Western blot50 mM Tris-HCl, 150 mM NaCl, pH 7.5other applications100 mM Tris-HCl, 150 mM NaCl, pH 7.51% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.Storage conditions (working solution): Diluted conjugate is stable at 2 to 8 °C for 12 hours.Always prepare freshly!

Specificity

• The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.Heat inactivation: yes

Application

• Use Anti-Digoxigenin-AP, Fab fragments for the detection of digoxigenin-labeled compounds using: • cDNA array• Colony/plaque hybridization• Dot blot• ELISA• Gel shift assay^[]• Immunohistocytochemistry^[]• In situ hybridization^[7]• Nonradioactive DNA sequencing blot• Northern blot^[]• RNase protection assay• Southern blot• Western blot

性质




Related Categories	DE-DL, 一抗, 字母顺序索引, 抗体
biological source	from sheep
antibody form	purified immunoglobulin
	purified immunoglobulin
clone	polyclonal
form	solution
packaging	pkg of 200 μL (150 U)
mfr. no.	Roche
isotype	IgG
conjugate	alkaline phosphatase conjugate
shipped in	wet ice
storage temp.	2-8°C







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