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Overview

IntestiCult™ Organoid Growth Medium (Mouse) is a defined, serum-free cell culture medium for efficient establishment and long-term maintenance of mouse intestinal organoids. Intestinal organoids, or "mini-guts", provide a convenient in vitro organotypic culture system for studying the intestinal epithelium and associated stem cell dynamics. The organoids feature a polarized epithelium containing all of the known cell types of the adult intestinal epithelium. They also incorporate a functional lumen and the crypt-villus structure that characterizes the in vivo intestine. Intestinal crypts rapidly form organoids when cultured in IntestiCult™ Organoid Growth Medium (Mouse). Applications of these cultures include studying the development and function of normal and tumorigenic intestinal epithelium, modelling intestinal disease and investigating stem cell properties and regenerative therapy approaches. Organoid culture enables in vitro characterization of a system with strong physiological relevance to the adult intestine.