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Stemcell  04447  MethoCult™ Express

Product Overview

MethoCult™ Express is intended for use in hematopoietic colony-forming unit (CFU) assays of human cord blood (CB) samples, after a minimum culture period of seven days. It is recommended for use with red blood cell depleted CB samples, whole CB samples that have been cryopreserved and thawed, and CB mononuclear cells. MethoCult™ Express is optimized for the growth and enumeration of human hematopoietic progenitor cells after much shorter periods than the 14 -16 days of standard CFU assays. In MethoCult™ Express, colonies containing at least 20 cells can be counted as early as after 7 days of culture. At this time, most colonies are immature and have not yet differentiated into morphologically distinguishable colony types. Therefore the colonies counted after seven days of culture give information about the total frequency of hematopoietic progenitor cells present in the sample without distinction between different progenitor types. If MethoCult™ Express cultures are maintained for 14 - 16 days, colonies derived from erythroid progenitors (BFU-E), granulocyte-macrophage progenitors (CFU-GM, CFU-G and CFU-M) and multi-potential granulocyte, erythroid, macrophage and megakaryocyte progenitors (CFU-GEMM) can be counted. MethoCult™ Express is registered as an In Vitro Diagnostic (IVD) device in select regions.

Contains:• Methylcellulose in Iscove's MDM

• Fetal bovine serum

• Bovine serum albumin

• Cytokines, including erythropoietin (EPO)

• Supplements

Subtype:半固体培养基; 专业培养基

Cell Type:造血干细胞和祖细胞

Species:人

Application:细胞培养; 集落检测; 功能检测; 体外诊断

Brand:MethoCult

Area of Interest:脐带血库; 干细胞生物学; 移植研究

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