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Stemcell  05742  NeuroCult™ NCFC Assay Kit (Rat)

Product Overview

The single-step Neural Colony-Forming Cell (NCFC) Assay allows discrimination between neural stem and progenitor cells. Using the NeuroCult™ NCFC Assay Kit (Rat), clonally-derived colonies of different sizes are generated and colony size is used to enumerate neural stem cells, as their proliferative potential is greater than that of progenitors and more mature cells. Cells with high proliferative potential form the largest sized colonies (greater than 2 mm in diameter) in the NCFC Assay and fulfill all the functional criteria of a neural stem cell. Cells with low and limited proliferative potential form colonies less than 2 mm in diameter. They are designated as progenitor cells and do not meet all the functional criteria of a stem cell.

NOTE: Addition of rh EGF (Catalog #78006), rh bFGF (Catalog #78003) and heparin (Catalog #07980) is required.

Components:

NeuroCult™ NCFC Assay Kit (Rat) (Catalog #05742)

NeuroCult™ NCFC Serum-Free Medium without Cytokines, 50 mL

NeuroCult™ NS-A Proliferation Supplement (Rat), 50 mL (Catalog #05773)

NeuroCult™ NS-A Basal Medium (Rat), 450 mL (Catalog #05770)

Collagen Solution, 35 mL (Catalog #04902

NeuroCult™ Collagenase Solution, 5 mL

35 mm Culture Dishes, 70 dishes (Catalog #27100)

60 mm Griddes Scoring Dishes, 5 dishes (Catalog #27500)

Subtype:半固体培养基; 专业培养基

Cell Type:神经干细胞和祖细胞

Species:大鼠

Application:细胞培养; 集落检测; 功能检测; 细胞球培养; 毒性检测

Brand:NeuroCult

Area of Interest:药物发现和毒性测试; 神经科学; 干细胞生物学

Formulation:成分确定; 无血清

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