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Stemcell 18162RF EasySep™ Human Th17 Cell Enrichment Kit
Product Overview
The EasySep™ Human Th17 Enrichment Kit is a two-step method designed to enrich human Th17 cells from fresh peripheral blood nucleated cells. CD4+CXCR3- T cells are first pre-enriched by negative selection using EasySep™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes (TAC) recognizing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, glycophorin A, CD45RAhigh, CXCR3 and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Following pre-enrichment, CCR6 positive CD4+CXCR3- T cells are selected using EasySep™ Human CCR6 Positive Selection Cocktail. Cells are targeted with TAC recognizing CCR6 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet and remain in the tube while the unwanted cells are poured off.
Advantages:• Fast and easy-to-use
• Up to 94% purity
• No columns required
Components:
EasySep™ Human Th17 Cell Enrichment Kit (Catalog #18162)
EasySep™ Human CD4+CXCR3- Pre-Enrichment Cocktail, 1 mL
EasySep™ Magnetic Particles, 2 x 1 mL
EasySep™ Human CCR6 Positive Selection Cocktail, 0.5 mL
EasySep™ Magnetic Nanoparticles, 1 mL
RoboSep™ Human Th17 Cell Enrichment Kit (Catalog #18162RF)
EasySep™ Human CD4+CXCR3- Pre-Enrichment Cocktail, 1 mL
EasySep™ Magnetic Particles, 2 x 1 mL
EasySep™ Human CCR6 Positive Selection Cocktail, 0.5 mL
EasySep™ Magnetic Nanoparticles, 1 mL
RoboSep™ Buffer (Catalog #20104) x 2
RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:细胞分选试剂盒
Cell Type:T细胞; CD4+T细胞
Species:人
Sample Source:白细胞去除术; PBMC
Selection Method:正选
Application:细胞分离
Brand:EasySep; RoboSep
Area of Interest:免疫学
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