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Stemcell  15361  Complete Kit for Human CD4+CD127lowCD25+ Regulatory T Cells

Product Overview

The Complete Kit for Human CD4+CD127lowCD25+ Regulatory T Cells is a simple two-step method that is designed to isolate CD4+CD127lowCD25+ cells from whole blood or buffy coat. CD4+CD127low T cells are enriched from whole blood or buffy coat by negative selection using RosetteSep™ CD4+CD127low T Cell Pre-Enrichment Cocktail. The RosetteSep™ cocktail contains a combination of antibodies that is directed against non-CD4+CD127low T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified cells are present as an enriched population at the interface between the plasma and the buoyant density medium. The enriched cells are then selected for CD25+ cells by positive selection using EasySep™ Human CD25 Positive Selection Kit. Cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

Advantages:• Fast and easy-to-use

• Up to 91% purity

• No columns required

• Can be combined with SepMate™ for consistent, high-throughput sample processing

Components:

RosetteSep™ Human CD4+CD127low T Cell Enrichment Cocktail (Catalog #15361)

RosetteSep™ Human CD4+CD127low T Cell Pre-Enrichment Cocktail, 5 x 2 mL

Complete Kit for Human CD4+CD127lowCD25+ Regulatory T Cells (Catalog #15861)

RosetteSep™ Human CD4+CD127low T Cell Pre-Enrichment Cocktail, 5 x 2 mL

EasySep™ Human CD25 Positive Selection Cocktail, 1 mL

EasySep™ Magnetic Particles, 1 mL

RoboSep™ Kit for Human CD4+CD127lowCD25+ Regulatory T Cells with Filter Tips (Catalog #15861RF)

RosetteSep™ Human CD4+CD127low T Cell Pre-Enrichment Cocktail, 5 x 2 mL

EasySep™ Human CD25 Positive Selection Cocktail, 1 mL

EasySep™ Magnetic Particles, 1 mL

RoboSep™ Buffer (Catalog #20104)

RoboSep™ Filter Tips (Catalog #20125)

Magnet Compatibility:

• EasySep™ Magnet (Catalog #18000)

• “The Big Easy” EasySep™ Magnet (Catalog #18001)

• RoboSep™-S (Catalog #21000)

Subtype:细胞分选试剂盒

Cell Type:T细胞; CD4+T细胞; 调节性T细胞

Species:人

Sample Source:白膜层; 全血

Selection Method:正选

Application:细胞分离

Brand:EasySep; RoboSep; RosetteSep

Area of Interest:免疫学

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