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Stemcell 15862 Complete Kit for Human CD4+CD25+ T Cells
Product Overview
The Complete Kit for Human CD4+CD25+ Regulatory T Cells is a simple two-step method that is designed to isolate CD4+CD25+ cells from whole blood or buffy coat. CD4+ T cells are enriched from whole blood or buffy coat by negative selection using RosetteSep™ CD4+ T Cell Enrichment Cocktail. The RosetteSep™ cocktail contains a combination of antibodies that is directed against non-CD4+ T cells and glycophorin A red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The enriched CD4+ T cells are then selected for CD25+ cells by positive selection using EasySep™ Human CD25 Positive Selection Kit. Cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Components:
Complete Kit for Human CD4+CD25+ T Cells (Catalog #15862)
RosetteSep™ Human CD4+ T Cell Enrichment Cocktail, 5 x 2 mL
EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
EasySep™ Magnetic Particles, 1 mL
RoboSep™ Kit for CD4+CD25+ T Cells with Filter Tips (Catalog #15862RF)
RosetteSep™ Human CD4+ T Cell Enrichment Cocktail, 5 x 2 mL
EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
EasySep™ Magnetic Particles, 1 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:细胞分选试剂盒
Cell Type:T细胞; CD4+T细胞; 调节性T细胞
Species:人
Sample Source:白膜层; 全血
Selection Method:正选
Application:细胞分离
Brand:EasySep; RoboSep; RosetteSep
Area of Interest:免疫学
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