MesenCult™-XF Medium

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MesenCult™-XF Medium
Defined, xeno-free medium for human mesenchymal stem cells
产品描述

MesenCult™-XF Medium is a standardized, xeno-free, serum-free medium for the culture of human mesenchymal stem cells (MSCs). MesenCult™-XF Medium is optimized for the expansion of mesenchymal stem cells in vitro as well as their enumeration using the colony-forming unit - fibroblast (CFU-F) assay. MesenCult™-XF Medium supports long-term growth of MSCs and cells maintain multi-lineage differentiation potential. MesenCult™-XF Medium must be used in conjunction with the MesenCult™-SF Attachment Substrate (Catalog #05424) and the MesenCult™-ACF Dissociation Kit (Catalog #05426). Components of the MesenCult™-SF Attachment Substrate and the MesenCult™-ACF Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-XF Medium. MesenCult™-XF Medium must also be supplemented with L-glutamine.

NOTE: MesenCult™-XF Medium and MesenCult™-SF Attachment Substrate are also available as part of the MesenCult™-SF Culture Kit (Catalog #05429).

Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages

Mesenchymal Stem and Progenitor Cell Research

Cell Sourcing and Isolation →Culture and Cryopreservation →Differentiation →Characterization

Data and Publications

Data

Figure 1. Human bone marrow-derived MSCs cultured in MesenCult™-XF expand faster than cells cultured in a traditional serum-based medium (n = 3, Mean ± SEM)

Figure 2. Human bone marrow-derived MSCs cultured in MesenCult™-XF display multi-lineage differentiation potential
(A) Oil Red O staining of adipocytes generated from passage 1 MSCs previously cultured in MesenCult™-XF. (B) Alizarin Red detection of Ca²⁺ deposits indicates the formation of bone structures in cells generated from passage 4 MSCs previously cultured in MesenCult™-XF. (C) Alcian Blue staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult™-XF. (D) Collagen II staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult™-XF.

Figure 3. Primary human bone marrow-derived MSCs show less hematopoietic cell contamination when cultured in (A) MesenCult™-XF compared to a (B) traditional serum-based medium
Passage 0 human bone marrow-derived MSCs cultured in MesenCult™-XF (A) or a traditional serum-based medium (B).

Figure 4. Phenotype of culture-expanded human MSCs cultured in MesenCult™-XF

Figure 5. MSCs cultured in MesenCult™-XF suppress T cell proliferation and reduce cell cycle division more robustly than MSCs cultured in a traditional serum-based medium
Passage 2 MSCs generated in MesenCult™-XF or a traditional serum-based medium were treated with mitomycin C prior to co-culture with T cells. T cells were purified from peripheral blood using EasySep™ (Catalog #19051) immunomagnetic separation and fluorescently labeled using 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (5(6)CFDA,SE, CFSE). 2 x 10e5 CFSE-labeled T cells were cultured with 1 x 10e5 MSCs in serum-free medium supplemented with 100 U/mL IL-2. T cells were stimulated with tetrameric antibody complexes against CD3ε, CD28 and CD2. On days 3 and 7, cells were harvested, stained with anti-CD45 antibody and propidium iodide and the T cell division history measured as CFSE dye dilution analyzed by flow cytometry.
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