重组人PD-L1蛋白(Recombinant Human PD-L1 Protein),GMP级
产品货号:GMP-TL727
产品描述(Description)
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
分子别名(Synonym)
CD274、B7-H1、PDCD1L1、PDCD1LG1
表达宿主(Expression host)
HEK293细胞
来源(Source)
Human
蛋白序列(Protein Sequence)
DNA序列编码人PD-L1(NCBI Reference Sequence:NP_054862.1)表达带有His标签在C末端
分子量(Molecular Weight)
重组人PD-L1蛋白 包含222个氨基酸,预测理论分子量为26.9 kD
纯度(Purity)
≥95%采用SDS-PAGE凝胶和高效液相色谱分析
内毒素(Endotoxin)
<0.1EU/ug
生物活性(Biological Activity)
固定 PD-L1(His 标签)1ug/mL(100 uL/孔)能够结合抗人PD-L1抗体的线性范围是0.5-5 ng/mL
稳定性(Stability)
冻干制剂可在 4℃保存 24 个月, 溶解后的液体可置于-20℃保存 6-12 个月, 避免反复冻融
组成(Component)
含有6%甘露醇的无菌PBS(PH 7.4)的冻干粉
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