重组小鼠 CD4蛋白(Recombinant Murine CD4 Protein)
产品货号:R-TL751
产品描述(Description)
T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
同义词(Alternative Names)
L3T4 Protein, Mouse、 Ly-4 Protein, Mouse
蛋白序列(Protein Sequence)
DNA 序列编码小鼠 CD4(UniProtKB/Swiss-Prot: P06332)表达带有 His 标签在 C 末端
分子量(Molecular Weight)
重组小鼠 CD4 蛋白包含 381 个氨基酸,预测理论分子量为 42.9kD
表达宿主(Expression host)
HEK293细胞
纯度(Purity)
>90%,采用 SDS-PAGE 凝胶分析
内毒素(Endotoxin)
<1EU/μg
纯化方式( Purification Method )
层析纯化
性状(Character)
白色疏松体
使用说明(Instructions)
冻干制剂可在 2~8℃保存 24 个月。
如需分装,可用注射用水、生理盐水、培养基或 PBS 溶解,溶解后分装成小份,置于-20℃保存 期 6 个月,-80℃保存期 12 个月,稀释后浓度不低于 100μg/mL。避免反复冻融。
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