重组人 Fetuin A/AHSG(Recombinant Human Fetuin A/AHSG),GMP级
产品货号:GMP-TL758
产品描述(Description)
Human Fetuin A, also known as alpha 2 -Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors. It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages . The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain . It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection. It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification . It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins. Recombinant Human Fetuin A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. The calculated molecular weight of Recombinant Human Fetuin A is 32.9 kDa. Due to glycosylation, recombinant Fetuin A migrates at an apparent molecular weight of 45–55 kDa by SDS-PAGE run under non-reducing conditions.
分子别名(Synonym)
Alpha-2-HS-glycoprotein、Alpha-2-Z-globulin、Ba-alpha-2-glycoprotein、Human AHS Protein、Human FETUA Protein、Human HSGA Protein
表达宿主(Expression host)
人HEK293 细胞
蛋白序列(Protein Sequence)
DNA 序列编码人 Fetuin A/AHSG(UniProtKB/Swiss-Prot: P02765)表达带有 His 标签在 C 末端
分子量(Molecular Weight)
重组人 Fetuin A/AHSG蛋白 包含 381 个氨基酸,预测的理论分子量为 41kd,还原性 SDS-PAGE 的分子量为58kd
纯度(Purity)
≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析
内毒素(Endotoxin)
≤0.01EU/ug(凝胶法)
储存条件(Storage)
冻干制剂可在 4℃保存 24 个月,溶解后的液体可置于-20℃保存 6-12 个月,避免反复冻融
纯化方式( Purification Method )
层析纯化
性状(Character)
本品为白色疏松体
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