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UltraGRO™-Advanced Research grade
Product Type:Fibrinogen depleted cell culture supplement
Attribute:non-xenogeneic (original material)
Grade:Research grade
Product Name:UltraGRO™-Advanced
Catalog Number:HPCFDCRL50, HPCFDCRL10, HPCFDCRL05
Description:
UltraGRO™-Advanced cell culture supplement is a fibrinogen depleted, non-xenogeneic (original material), animal serum-free supplement for replacing FBS (fetal bovine serum) to support cell expansion from research use. UltraGRO™-Advanced contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale production.
Quality Control Test:
1. Appearance: Yellow to Buff liquid
2. Total Protein: 4.0 - 8.0 g/dL
3. Endotoxin: ≤ 10 EU/mL
4. Sterility: Pass
5. Mycoplasma: Negative
6. pH: Tested
7. Osmolality: Tested
8. Performance Test: Test and record
Storage and Shipping Information:
UltraGRO™-Advanced is most stable when stored frozen until needed. The recommended storage temperature is -20°C. Thaw frozen UltraGRO™-Advanced product in 37°C water bath before use. Once UltraGRO™-Advanced is thawed, it is recommended to fully use for completed medium preparation (e.g. 5%) the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C.
Packaging Information:
500mL, 100mL, 50mL plastic bottles
Media Form:
Frozen Liquid
Application:
For human ex-vivo tissue and cell culture processing applications.
Instructions for Use:
1. UltraGRO™-Advanced shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.
2. We recommend seeding MSCs at approximately 3x103~6x103 per cm2.
3. UltraGRO™-Advanced has been fibrinogen-depleted and does not require the addition of heparin in the cell culture media.
Cell Lines:
bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells.
Important Information:
1. Insoluble particles may form in thawed UltraGRO™-Advanced. Published research has shown that particles will not alter the performance of the product.
2. Insoluble particles may form in thawed UltraGRO™-Advanced, it is recommended to centrifuge at 3,400 xg for 3~5 minutes.
3. Filtering the completed medium (e.g. 5%), after UltraGRO™-Advanced is diluted in the basal medium, will not affect UltraGRO™-Advanced supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™-Advanced concentrate, as this may reduce 5% UltraGRO™ -Advanced cell culture performance.
4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™-Advanced performance.
Safety information:
1. Follow the handling instructions outlined in the Material Safety Date Sheets (MSDSs). Wear appropriate protective eyewear, clothing, and gloves.
2. UltraGRO™-Advanced is a cell culture supplement collected from healthy donors at FDA licensed blood centers. Each donor has been tested using FDA licensed tests and found nonreactive for HBsAg, Hepatitis B core antibody (anti-HBc), HIV antibody (anti-HIV-1/2), Hepatitis C antibody (anti-HCV), HTLV-1/2 antibody (anti-HTLV-1/2), Trypanosoma cruzi antibody (anti-T. cruzi), HIV-1, HCV, HBV, WNV nucleic acid testing and Syphilis microhemagglutination test.
3. This product is for in vitro use only.
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