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Sigma-Aldrich  P8340  Protease Inhibitor Cocktaill蛋白酶抑制剂

产品编号 P8340 | SIGMA

for use with mammalian cell and tissue extracts, DMSO solution

产品名称

Components

AEBSF

Aprotinin

Bestatin

E-64

Leupeptin

Pepstatin A

Application

Optimized and tested for general use.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.

Caution

This cocktail should be stored at -20 °C.

包装

1, 5 mL in serum bottle

Preparation Note

One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.

Specificity

Inhibits serine, cysteine, and acid proteases, and aminopeptidases.

性质

Related Categories：Core Bioreagents, Enzyme Inhibitors, HIS Select Supporting Products and Reagents, HIS-Select, Life Science Reagents for Protein Expression and Purification,Nanodisc Essentials, Nanodisc Reagents for Membrane Protein Research, Products for UpStream Processes, Protease Inhibitor Cocktails for HIS-Select, Protease Inhibitors, Research Essentials, 分子生物学, 生化试剂, 纯化和检测, 蛋白质及衍生物, 蛋白质组学, 蛋白酶抑制剂Cocktails, 酶、抑制剂和底物, 重组蛋白表达和分析

Quality Level：PREMIUM

form：DMSO solution

storage temp.：−20°C

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