Clone: | Poly19010 (See other available formats) |
Isotype: | Rabbit Polyclonal |
Reactivity: | Human, Mouse, Chicken, Xenopus, Zebrafish |
Immunogen: | This Pax-2 antibody was generated against a recombinant protein containing 22kD of the Pax-2 sequence corresponding to amino acids 188-385. |
Formulation: | Phosphate-buffered solution + 0.03% Thimerosal. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 1 mg/ml |
Storage & Handling: | The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. |
Application: | IHC, WB, IP |
Recommended Usage: | Each lot of this antibody is quality control tested by immunohistochemical staining.The optimal working dilution should be determined for each specific assay condition. • WB: 1:250-1,000*• Gel shifts: 1:10• IHC: 1:100Tissue Sections: Formalin-fixed, paraffin-embedded tissues, acetone or PFA fixed frozen sectionsPretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieva-All 1 (Cat. No. 927901).Incubation: 20 minutes at room temperatureStaining Localization: nuclear |
ApplicationNotes: | This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunopurification and DNA band supershift experiments.*Predicted MW = 44 kDPositive Control Tissue: normal kidney or renal adenocarcinomaThis antibody recognizes Pax-2A and Pax-2B (two products of the Pax-2 gene). When linked to agarose beads, it has been used to immunoprecipitate DNA sequences recognized by Pax-2. |
ApplicationReferences: | 1. Hammes A, et al. 2001. Cell. 106:319.2. Phelps DE, Dressler GR. 1996. J Biol Chem. 271:7978.3. Rothenpieler UW, Dressler GR. 1993. Development. 119:7114. Dressler GR, et al. 1993. Nature. 362:655. Dressler GR, Douglass EC. 1992. Proc Natl Acad Sci USA.89:11796. Puschel AW, et al. 1992. Mech Dev. 38:1977. Riley BB, et al. 1999. Development. 126:5669. (IHC)8. Huang L, et al. 2016. Neoplasia. 18:71-81. PubMed9. Zhao F, et al. 2016. Sci Rep. 6:23037. PubMed10. Prunskaite-Hyyryläinen R, et al. 2016. Hum. Mol. Genet.. 25: 1059 - 1073. PubMed11. Ohnmacht J, et al. 2016. Development. 143: 1464 - 1474.PubMed12. Dauleh S, et al. 2016. PLOS ONE. 11: 0158997. PubMed |
Publication Library | |
RRID: | AB_2565001 (BioLegend Cat. No. 901001) |
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