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Biolegend(Covance) 808401 Purified anti-Myelin Basic Protein Antibody
产品编号 808401| Biolegend(Covance)
产品描述
Clone: | SMI 99 (See other available formats) |
Isotype: | Mouse IgG2b |
Reactivity: | Mammalian |
Formulation: | Phosphate-buffered solution + 0.01% sodium azide. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 1 mg/ml |
Storage & Handling: | The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. |
Application: | IHC, WB, ICC, ELISA |
Recommended Usage: | Each lot of this antibody is quality control tested by immunohistochemical staining.The optimal working dilution should be determined for each specific assay condition.• WB: 1:1,000• ICC: 1:1,000• IHC: 1:2,000-1:4,000Tissue Sections: Formalin-fixed, paraffin-embedded tissues & frozen sectionsPretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 3 pH4.8 (Cat. No. 927601). Incubation: 60 minutes at room temperature with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501). The extent of permissible dilution of SMI-99 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation, and kind of section examined. |
ApplicationNotes: | This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC), immunohistochemistry (IHC), and ELISA.Positive Control: Human cerebellum tissueThis antibody reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of the classic human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. Pig and chicken MBP do not react and guinea pig MBP has slight reactivity. The antibody does not react with the 14 kD form of rat MBP. This antibody detects developing and adult myelin, developing oligodedrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections. The combination of this antibody with clone SMI-91 and/or SMI-94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.This antibody is DEAE-purified IgG from mouse ascites. |
ApplicationReferences: | 1. Fyffe-Maricich S, Karlo J, Landreth G, Miller R. The ERK2 Mitogen-Activated protein kinase regulates the timing of oligodendrocyte differentiation. J. Neurosci.; 31: 843 - 850, 2011.[IHC, WB] PubMed 2. Mendz GL, Moore WJ, Easterbrook-Smith S, Linthicum DS. Proton-n.m.r. study of interaction of myelin basic protein with a monoclonal antibody. Biochem J 228:61-68, 1985.3. DeRosbo NK, Carnegie PR, Bernard CCA, Linthicum DS. Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting. Neurochem Res 9:1359-1369, 1984.4. Carnegie PR, Dowse CA, Linthicum DS. Antigenic determinant recognized by a monoclonal antibody to human myelin basic protein. J Neuroimmunol 5:125-134, 1983.5. Dowse CA, Carnegie PR, Linthicum DS, Bernard CCA. Solid phase radioimmunoassay for human myelin basic protein using a monoclonal antibody. J Neuroimmunol 5:135-144, 1983. 6. Del Valle L,et al. Cancer Biol Ther.9:286. (IHC) PubMed 7. Kiryu-Seo S, Ohno N, Kidd GJ, Komuro H, Trapp BD. Demyelination Increases Axonal Stationary Mitochondrial Size and the Speed of Axonal Mitochondrial Transport. J. Neurosci 30: 6658-6666, May 2010 (ICC) PubMed 8. Samanta J, Alden T, Gobeske K, Kan L, and Kessler JA. Noggin Protects Against Ischemic Brain Injury in Rodents. Stroke, 41: 357-362, Feb 2010. (IHC) PubMed 9. Werner HB, et al. Proteolipid Protein Is Required for Transport of Sirtuin 2 into CNS Myelin. J. Neurosci., 27: 7717-7730, Jul 2007.(WB, ICC) PubMed 10. Liu J, et al. 2016. J. Neurosci.. 36: 957 - 962. PubMed11. Hernandez M, et al. 2016. J. Neurosci.. 36: 806 - 813. PubMed12. Luo F, et al. 2016. J. Neurosci.. 36: 3024 - 3037. PubMed |
Publication Library | |
RRID: | AB_2564741 (BioLegend Cat. No. 808401)AB_2564742 (BioLegend Cat. No. 808402) |
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Covance主要业务是研发和生产各种抗体，涉及领域包括：神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。特色产品是免疫组化的体外诊断试剂。美国 BioLegend 公司于圣地亚哥时间2014年8月29日收购了位于马萨诸塞州戴德姆的科文斯（Covance）旗下的科文斯抗体服务公司（Covance Antibody Services Inc.）。科文斯抗体服务公司主要业务是研发和生产各种抗体，涉及领域包括:神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。这些用于免疫组化的体外诊断试剂（IVD）对于 BioLegend 公司目前的科研试剂产品线是一个很好的补充和扩展。科文斯抗体的客户将享有与 BioLegend 当前客户同样的优质服务和高性价比的产品。
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