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Biolegend(Covance) 801701 Purified anti-Neurofilament H (NF-H), Nonphosphorylated Antibody
产品编号 801701| Biolegend(Covance)
产品描述
Clone: | SMI 32 (See other available formats) |
Isotype: | Mouse IgG1 |
Reactivity: | Mammalian, Mouse |
Formulation: | Phosphate-buffered solution. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 1 mg/ml |
Storage & Handling: | The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. |
Application: | IHC, WB, ICC, ELISA |
Recommended Usage: | Each lot of this antibody is quality control tested by immunohistochemical staining.The optimal working dilution should be determined for each specific assay condition.• WB: 1:1,000*• IHC: 1:2,500-1:5,000Tissue Sections: Formalin-fixed, paraffin-embedded tissues & frozen sectionsPretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 3 pH4.8 (Cat. No. 927601). Incubation: 60 minutes at room temperature with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501). • ELISA: 1:1,000The extent of permissible dilution of SMI 32 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation, and kind of section examined. |
ApplicationNotes: | This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunocytochemistry and ELISA.*SMI 32 visualizes two bands (200 and 180 kD) which merge into a single NFH line on two-dimensional blots. Positive Control: Human cerebellum tissueThis antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+ uptake. |
ApplicationReferences: | 1. Chang Q and Martin LJ. Glycine Receptor Channels in Spinal Motoneurons Are Abnormal in a Transgenic Mouse Model of Amyotrophic Lateral Sclerosis. J. Neurosci., 31: 2815-2827, Feb 2011. [ICC] PubMed 2. Stevens HE, Smith KM, Maragnoli ME, Fagel D, Borok E, Shanabrough M, Horvath TL, and Vaccarino FM. Fgfr2 Is Required for the Development of the Medial Prefrontal Cortex and Its Connections with Limbic Circuits. J. Neurosci., 30: 5590-5602, Apr 2010. [ICC] PubMed 3. Trapp BD, Peterson J, Ransohoff RM, Rudick R, Mörk S, Bö L. Axonal transection in the lesions of multiple sclerosis. N Eng J Med 338:278-85, 1998.4. King CE, Jacobs I, Dickson TC, Vickers JC. Physical damage to rat cortical axons mimics early Alzheimer's neuronal pathology.Neuroreport 8:1663-5, 1997.5. Campbell MJ, Hof PR, Morrison JH. A subpopulation of primate cortical neurons is distinguished by somatodendritic distribution of neurofilament protein. Brain Res 539:133, 1991.6. Campbell MJ, Morrison J. Monoclonal antibody to neurofilament protein (SMI 32) labels a subpopulation of pyramidal axons in human and monkey neocortex. J Comp Neurol 282:191, 1990.7. Sternberger LA, Sternberger NH. Monoclonal antibodies distinguish phosphorylated and non-phosphorylated forms of neurofilaments in situ. PNAS USA 80:6126-30, 1983. 8. Petzold A, et al. 2011. Brain 134:464. (WB) PubMed 9. Kiryu-Seo S, Ohno N, Kidd GJ, Komuro H, Trapp BD. Demyelination Increases Axonal Stationary Mitochondrial Size and the Speed of Axonal Mitochondrial Transport. J. Neurosci 30: 6658-6666, May 2010 (ICC) PubMed 10. Redondo J, et al. 2014. Brain Pathol. doi: 10.1111/bpa.12230.(IF) PubMed 11. Turner M, et al. 2015. Journal of Neuroimmunology. 285: 4-12.PubMed12. Pagliarini V, et al. 2015. J. Cell Biol.. 211: 77 - 90. PubMed |
Publication Library | |
RRID: | AB_2564642 (BioLegend Cat. No. 801701) |
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