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Biolegend(Covance) 901503 Purified anti-HA.11 Epitope Tag Antibody
产品编号 901503| Biolegend(Covance)
产品描述
Clone: | 16B12 (See other available formats) |
Isotype: | Mouse IgG1, κ |
Isotype Control: | Purified Mouse IgG1, κ Isotype Ctrl |
Reactivity: | YPYDVPDYA Tag |
Immunogen: | Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL. |
Formulation: | Phosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 1 mg/ml |
Storage & Handling: | Store between 2°C and 8°C. |
Application: | WB, IF, IP |
Recommended Usage: | Each lot of this antibody is quality control tested by Western blotting.The optimal working dilution should be determined for each specific assay condition. • WB: 1:1,000*• IF: 1:1,000• IP: 1:150 |
ApplicationNotes: | This antibody is effective in immunoblotting (WB), immunofluorescence (IF), and immunoprecipitation (IP) of tagged proteins.*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. |
ApplicationReferences: | 1. Pecot MY, Malhotra V. 2004. Cell. 116:99. 2. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB) 3. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB) 4. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB) 5. Kolodziej P, Young R. 1991. Meth Enzymol. 194:508. (General) 6. Field J, et al. 1988. Mol Cell Biol. 8:2159. (General) 7. Majeed SR, et al. 2014. Nat Commun. 23:5891. (IF) PubMed 8. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed 9. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed 10. Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed 11. Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed12. Block E, et al. 2015. J. Neurosci.. 35: 12845-12858. PubMed |
Publication Library | |
RRID: | AB_2565006 (BioLegend Cat. No. 901501)AB_2565007 (BioLegend Cat. No. 901502)AB_2565005 (BioLegend Cat. No. 901503) |
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公司简介
Covance主要业务是研发和生产各种抗体，涉及领域包括：神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。特色产品是免疫组化的体外诊断试剂。美国 BioLegend 公司于圣地亚哥时间2014年8月29日收购了位于马萨诸塞州戴德姆的科文斯（Covance）旗下的科文斯抗体服务公司（Covance Antibody Services Inc.）。科文斯抗体服务公司主要业务是研发和生产各种抗体，涉及领域包括:神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。这些用于免疫组化的体外诊断试剂（IVD）对于 BioLegend 公司目前的科研试剂产品线是一个很好的补充和扩展。科文斯抗体的客户将享有与 BioLegend 当前客户同样的优质服务和高性价比的产品。
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