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Biolegend(Covance) 802001 Purified anti-Tubulin β-3 (TUBB3) Antibody
产品编号 802001| Biolegend(Covance)
产品描述
Clone: | Poly18020 (See other available formats) |
Isotype: | Rabbit Polyclonal |
Reactivity: | Mammalian |
Immunogen: | This antibody was generated against the same epitope as recognized by TUJ1 and was subsequently purified on a peptide affinity column. |
Formulation: | Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 1 mg/ml |
Storage & Handling: | Store between 2°C and 8°C. |
Application: | IHC, WB, IF, ICC |
Recommended Usage: | Each lot of this antibody is quality control tested by immunohistochemical staining.The optimal working dilution should be determined for each specific assay condition.• WB: 1:5,000*• ICC: 1:1,000-1:2,500• IHC: 1:5,000-10,000Tissue Sections: Formalin-fixed, paraffin-embedded tissues & frozen sectionsPretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (Cat. No. 928501, 928601). Incubation: 60 minutes at room temperature |
ApplicationNotes: | This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC).*Predicted MW = 50 kDThis antibody is well characterized and highly reactive to neuron specific Class III β-Tubulin (βIII), but does not identify β-tubulin found in glial cells. |
ApplicationReferences: | 1. Feuer R, Pagarigan RR, Harkins S, Liu F, Hunziker IP, Whitton JL. Coxsackievirus targets proliferating neuronal progenitor cells in the neonatal CNS. J. Neurosci. 25(9):2434-44, 2005.2. Mozzetti S, Ferlini C, Concolino P, Filippetti F, Raspaglio G, Prislei S, Gallo D, Martinelli E, Ranelletti FO, Ferrandina G, Scambia G. Class III beta-tubulin overexpression is a prominent mechanism of paclitaxel resistance in ovarian cancer patients. Clin. Cancer Res. 11(1):298-305, 2005.3. Feuer R, Mena I, Pagarigan RR, Harkins S, Hassett DE, Whitton JL. Coxsackievirus B3 and the neonatal CNS: the roles of stem cells, developing neurons, and apoptosis in infection, viral dissemination, and disease. Am. J. Pathol. 163(4):1379-93, 2003.4. Zhang XM, Yang XJ. Regulation of retinal ganglion cell production by Sonic hedgehog. Development 128(6):943-957, 2001. 5. Ma M, et al. 2012. Neurobiol Dis. 56:34. (WB) PubMed 6. Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed 7. Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC)PubMed 8. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF)PubMed 9. Macpherson P, et al. 2015. Development. 142: 4038 - 4048. PubMed10. Yau K, et al. 2016. J. Neurosci.. 36: 1071 - 1085.PubMed11. Chow A, Morshead C 2016. Stem Cell Res. 16:79-87.PubMed12. Cui Q, et al. 2016. Nat Commun. 7:10637. PubMed |
Publication Library | |
RRID: | AB_2564645 (BioLegend Cat. No. 802001) |
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公司简介
Covance主要业务是研发和生产各种抗体，涉及领域包括：神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。特色产品是免疫组化的体外诊断试剂。美国 BioLegend 公司于圣地亚哥时间2014年8月29日收购了位于马萨诸塞州戴德姆的科文斯（Covance）旗下的科文斯抗体服务公司（Covance Antibody Services Inc.）。科文斯抗体服务公司主要业务是研发和生产各种抗体，涉及领域包括:神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。这些用于免疫组化的体外诊断试剂（IVD）对于 BioLegend 公司目前的科研试剂产品线是一个很好的补充和扩展。科文斯抗体的客户将享有与 BioLegend 当前客户同样的优质服务和高性价比的产品。
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