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Biolegend(Covance) 901301 Purified anti-Pax-6 Antibody
产品编号 901301| Biolegend(Covance)
产品描述
Clone: | Poly19013 (See other available formats) |
Isotype: | Rabbit Polyclonal IgG |
Reactivity: | Human, Mouse, Mammalian |
Immunogen: | This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein. |
Formulation: | Phosphate-buffered solution + 0.03% Thimerosal. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 2 mg/ml |
Storage & Handling: | The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. |
Application: | WB, IHC, IF |
Recommended Usage: | Each lot of this antibody is quality control tested by Western blotting and immunohistochemistry.The optimal working dilution should be determined for each specific assay condition.• Western blot: 1:200-500*• IHC: 1:50-1:100• IF: 1:300Tissue Sections: paraffin-embedded tissues, frozen sectionsPretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (928501, 928601).Incubation: 60 minutes at room temperature |
ApplicationNotes: | This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF) and staining of tissue sections.*Predicted MW = 47-50 kDPositive Control: human brain, epididymisThe sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes. |
ApplicationReferences: | 1. Tang K, et al. 2012. Development. 139:1630. (IF) PubMed 2. Bandah D, et al. 2007. Invest. Ophthalmol. Vis. Sci. 48:2503. (WB) PubMed 3. Marquardt T, et al. 2001. Cell. 105:43. 4. Yamamoto Y, Jeffery WR. 2000. Science. 289:631 5. Ashery-Padan R, et al. 2000. Genes Dev. 14:2701. 6. Osumi N, et al. 1997. Development. 124:2961. 7. Koroma BM, et al. 1997. Invest. Ophthalmol Vis Sci. 38:108. 8. Davis JA, Reed RR. 1996. J Neurosci. 16:5082. (IHC)9. Small K, et al. 2015. Ophthalmology. 13: 681-685. PubMed10. Ahmad Z, et al. 2015. PLOS ONE. 10: 0144597. PubMed11. Verheyen A, et al. 2015. PLOS ONE. 10: 0146127. PubMed12. Bando Y, et al. 2016. Cereb Cortex. 26: 106 - 117. PubMed |
Publication Library | |
RRID: | AB_2565003 (BioLegend Cat. No. 901301) |
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公司简介
Covance主要业务是研发和生产各种抗体，涉及领域包括：神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。特色产品是免疫组化的体外诊断试剂。美国 BioLegend 公司于圣地亚哥时间2014年8月29日收购了位于马萨诸塞州戴德姆的科文斯（Covance）旗下的科文斯抗体服务公司（Covance Antibody Services Inc.）。科文斯抗体服务公司主要业务是研发和生产各种抗体，涉及领域包括:神经生物学、免疫病理学、细胞生物学、表位抗体和免疫组化等方面的科研试剂。这些用于免疫组化的体外诊断试剂（IVD）对于 BioLegend 公司目前的科研试剂产品线是一个很好的补充和扩展。科文斯抗体的客户将享有与 BioLegend 当前客户同样的优质服务和高性价比的产品。
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