Cell Signaling(CST) 14822 PathScan® Cancer Phenotype Antibody Array Kit (Fluorescent Readout)

产品编号 14822 | Cell Signaling(CST)

Kit Includes	Quantity	Applications	Reactivity	Homology†	MW (kDa)	Isotype
Array Slides - Cancer Phenotype Array	1 Kit
Detection Antibody Cocktail (10X) - Cancer Phenotype Array	300 µl
16-Well Gasket	2 ea
PathScan^® Sandwich ELISA Lysis Buffer (1X) #7018	30 ml
20X Array Wash Buffer	15 ml
DyLight 680TM-linked Streptavidin (10X)	300 µl
Sealing Tape	2 sheets
Array Blocking Buffer	5 ml
Array Diluent Buffer	15 ml

PathScan^® Cancer Phenotype Antibody Array Kit (Fluorescent Readout) detects the target proteins as specified on the Array Target Map. No substantial cross-reactivity has been observed between targets. This kit is optimized for cell lysates diluted to a total protein concentration between 0.2 and 1 mg/ml (see kit protocol). All sandwich assays have been validated for human derived samples. This kit detects proteins from the indicated species as determined through in-house testing, but may also detect homologous proteins from other species.

The PathScan^® Cancer Phenotype Antibody Array Kit (Fluorescent Readout) uses glass slides as the planar surface and is based upon the sandwich immunoassay principle. The array kit allows for the simultaneous detection of 19 cancer cell associated proteins. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two slides allowing for the interrogation of 32 different samples and the generation of 608 data points in a single experiment. Cell lysates are incubated on the slide followed by a biotinylated detection antibody cocktail. DyLight™ 680-linked Streptavidin is then used to visualize the bound detection antibody. A fluorescent image of the slide can then be captured with a digital imaging system and spot intensities quantified using array analysis software.

Despite shared hallmarks and common overarching principles, cancers are heterogeneous in nature. Widely used tumor derived cell lines often exhibit both genomic and proteomic differences relative to one another. Factors contributing to these differences include gradual accumulation of genetic lesions within cell lines, transcriptional networks and epigenetic marks remaining from the original tissue, and various adaptations to in vitro culture conditions. Cell lines may differ considerably in the architectural details of basic machineries, the wiring of signal transduction circuitry, and the set points of metabolic pathways. As a result, heterogeneous phenotypes within cell lines will manifest as differences in fundamental cellular functions and a range of behaviors under experimental conditions.

Scheel, C. and Weinberg, R.A. (2012) Semin Cancer Biol 22, 396-403.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

CST公司是一家由研究型科学家创建于1999年的家族式私营企业，在全球范围内拥有超过400多名员工。CST活跃于应用系统生物学研究，尤其是与癌症相关的领域。作为一家始终致力于科学研究的公司，CST深知抗体的高特异性和批次间性能的一致性至关重要。因此我们坚持自己生产所有的抗体，并在多种应用中进行严格的验证。同时，正是生产这些抗体的CST科学家为客户提供技术支持，帮助他们设计实验，解决问题以获得最可靠的结果。我们之所以这样做，是因为如果我们做实验，我们也想要得到同样的服务。事实上，CST的确是这样的。
Uante,您的科研物资战略合作伙伴！

重庆市寰宇安泰生物科技有限公司

固定电话：023-67946738

全国热线：400-8623-068

市场价

￥0.00

价格

￥0.00

品牌

CST

型号

14822

规格

1 Kit

客服